The fluorescence of a protein is due to tryptophan, tyrosine, and phenylalanine (assuming that the protein...
The fluorescence of a protein is due to tryptophan, tyrosine, and phenylalanine (assuming that the protein does not contain a prosthetic group that is fluorescent). Iodine ions are known to quench the fluorescence of tryptophan. if a protein is known to contain only one tryptophan residue and iodide fails to quench its fluorescence, what can you conclude about location of the tryptophan residue?
Homework Answers
it conclude that tryptophan residue is not located on perpheri but is located in bulk or core of the protein where the entrance of Iodide is hindered
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The fluorescence of a protein is due to tryptophan, tyrosine,
and phenylalanine (assuming that the protein...
Access the fluorescence excitation and emission spectra for phenylalanine, tyrosine, and tryptophan using the Internet....
Access the fluorescence excitation and emission spectra for phenylalanine, tyrosine, and tryptophan using the Internet. Answer the following four questions. a. Estimate peak wavelengths for absorption (at λ> 230nm) and emission (at λ> 250nm) for each amino acid. b. How are the peak absorption wavelengths related to the peak emission wavelengths? For example, is the emitted light at lower or higher energy than the absorbed light? Briefly explain. c. Which amino acid shows the biggest energy change from absorption to...
ANSWE THE FOLLOWING QUESTIONS BASED ON THE TEXT ABOVE: 1) The amino acid tyrosine is synthesized...
ANSWE THE FOLLOWING QUESTIONS BASED ON THE TEXT ABOVE:
1) The amino acid tyrosine is synthesized from phenylalanine.
Does this make tyrosine an essential amino acid in indivduals with
PKU?
2) In addition to phenylalanine, high levels of what other
compound(s) might show up in the urine of those with PKU?
Why?
THE GENETIC REVOLUTION PKU Phenylketonuria (PKU), the recessively inherited genetic disorder of amino acid metabolism, is regarded as the gold-standard genetic disorder. Its discovery in 1935 laid the...
Ribosome is composed of RNA and protein and can be thought of as ________. a. a...
Ribosome is composed of RNA and protein and can be thought of as ________. a. a protein pool b. an immune booster c. a protein transportation machine d. a protein-making machine e. a template for protein synthesis Intestinal tripeptidases cleave ________. a. amino acids b. polypeptides c. oligopeptides d. dipeptides e. tripeptides Which is not a group of lipoproteins? a. Chylomicrons b. HDL c. VLDL In epigenetic study, methylation involves ________. a. the failure to metabolize tyrosine b. the removal...
Hagrid has purified a protein from unicom sweat and called UNI-1. He has then doned the...
Hagrid has purified a protein from unicom sweat and called UNI-1. He has then doned the Unit gone and inserted in rat zygotes (by microinjection) a recombinant construct containing the Uni-1 coding sequence its entire 5 UTR region, covering both the minimal promoter and a series of cutative regulatory als elements. The mutantrats develop glorious, luminous hair. Hagrid has also discovered that the UNI-1 protein binds very tightly to DNA and hypothesizes it is a transcription factor that regulates proliferation...
I need help figuring out the flowchart and if I have the right answers for the...
I need help figuring out the flowchart and if I have the right
answers for the prelab please and thank you!
Group I, the Chloride Group BACKGROUND The precipitating reagent for the chloride group is chloride ion in an acidic solution. Only three of the 18 metal ions form a precipitate with this reagent: silver ion (Ag'), mercury(I)ion (Hg). and lead ion (Pb). Since lead chloride is somewhat soluble, it is not completely precipitated here, and a low concentration of...
I need help with the prelab and the flowchart. I’m totally clueless on the flowchart. For...
I need help with the prelab and the flowchart. I’m totally
clueless on the flowchart.
For the prelab questions I have some answers but I don’t know
if they’re right. If they’re wrong, I would greatly appreciate an
explanation for the correct answer. I’m really trying to learn the
material but it’s hard now that classes are cancelled so I’m sorry
if these are dumb
1. PbCl ?
2. Ag+
3. ?
4. S1
Group 1, the Chloride Group BACKGROUND...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing a KDEL sequence (GFP-KDEL). When GFP-KDEL is expressed in normal human fibroblasts and examined using fluorescence microscopy, the fluorescence appears diffuse across the cytoplasm. How would you explain this observations given that KDEL is supposed to be an ER-specific sorting sequence? A. This engineered GFP would not have a hydrophobic signal sequence to get it into the RER in the first place. B. The...
Stat 5644 Spring 2019 Homework 4 Due 3/20 Problem :UMVUE via Rao-Blackwell, Lehmann-Scheffe, and ...
Stat 5644 Spring 2019 Homework 4 Due 3/20 Problem :UMVUE via Rao-Blackwell, Lehmann-Scheffe, and Basu theorems This problem is on the estimation of a reliability function. Let Xi, , x, be IID from N(μ, σ2). Let Φ(-) be the c.d.f. of the standard normal distribution. Assume that σ2-of is known, for now. It is of interest to estimate the reliability number σο for some c, with )-1-(). c is called cut of point in reliability (a) Give, without any proof,...
1 Overview and Background Many of the assignments in this course will introduce you to topics in ...
1 Overview and Background Many of the assignments in this course will introduce you to topics in computational biology. You do not need to know anything about biology to do these assignments other than what is contained in the description itself. The objective of each assignment is for you to acquire certain particular skills or knowledge, and the choice of topic is independent of that objective. Sometimes the topics will be related to computational problems in biology, chemistry, or physics,...
Use of Modern Molecular Techniques to Determine the Synthetic Pathway of a Novel Amino Acid. Most...
Use of Modern Molecular Techniques to Determine the Synthetic Pathway of a Novel Amino Acid. Most of the biosynthetic pathways described in our book were determined before the development of recombinant DNA technology and genomics, so the techniques were quite different from those that researchers would use today. Through this question, you will explore an example of the use of modern molecular techniques to investigate the pathway of synthesis of a novel amino acid, (2S)-4- amino-2-hydroxybutyrate (AHBA). AHBA is a...
ANSWE THE FOLLOWING QUESTIONS BASED ON THE TEXT ABOVE:
1) The amino acid tyrosine is synthesized from phenylalanine.
Does this make tyrosine an essential amino acid in indivduals with
PKU?
2) In addition to phenylalanine, high levels of what other
compound(s) might show up in the urine of those with PKU?
Why?
THE GENETIC REVOLUTION PKU Phenylketonuria (PKU), the recessively inherited genetic disorder of amino acid metabolism, is regarded as the gold-standard genetic disorder. Its discovery in 1935 laid the...
Hagrid has purified a protein from unicom sweat and called UNI-1. He has then doned the Unit gone and inserted in rat zygotes (by microinjection) a recombinant construct containing the Uni-1 coding sequence its entire 5 UTR region, covering both the minimal promoter and a series of cutative regulatory als elements. The mutantrats develop glorious, luminous hair. Hagrid has also discovered that the UNI-1 protein binds very tightly to DNA and hypothesizes it is a transcription factor that regulates proliferation...
I need help figuring out the flowchart and if I have the right
answers for the prelab please and thank you!
Group I, the Chloride Group BACKGROUND The precipitating reagent for the chloride group is chloride ion in an acidic solution. Only three of the 18 metal ions form a precipitate with this reagent: silver ion (Ag'), mercury(I)ion (Hg). and lead ion (Pb). Since lead chloride is somewhat soluble, it is not completely precipitated here, and a low concentration of...
I need help with the prelab and the flowchart. I’m totally
clueless on the flowchart.
For the prelab questions I have some answers but I don’t know
if they’re right. If they’re wrong, I would greatly appreciate an
explanation for the correct answer. I’m really trying to learn the
material but it’s hard now that classes are cancelled so I’m sorry
if these are dumb
1. PbCl ?
2. Ag+
3. ?
4. S1
Group 1, the Chloride Group BACKGROUND...
Stat 5644 Spring 2019 Homework 4 Due 3/20 Problem :UMVUE via Rao-Blackwell, Lehmann-Scheffe, and Basu theorems This problem is on the estimation of a reliability function. Let Xi, , x, be IID from N(μ, σ2). Let Φ(-) be the c.d.f. of the standard normal distribution. Assume that σ2-of is known, for now. It is of interest to estimate the reliability number σο for some c, with )-1-(). c is called cut of point in reliability (a) Give, without any proof,...
1 Overview and Background Many of the assignments in this course will introduce you to topics in computational biology. You do not need to know anything about biology to do these assignments other than what is contained in the description itself. The objective of each assignment is for you to acquire certain particular skills or knowledge, and the choice of topic is independent of that objective. Sometimes the topics will be related to computational problems in biology, chemistry, or physics,...
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