Homework Answers
Request Answer!
We need at least 10 more requests to produce the answer.
0 / 10 have requested this problem solution
The more requests, the faster the answer.
Add Answer to:
4. The complementation and recombination experiments both involve coinfection of E. coli cells with two different...
The following set of three separate experiments was conducted. Two strains of Escherichia coli are used....
The following set of three separate experiments was conducted. Two strains of Escherichia coli are used. Strain 1 needs histidine added to the medium to grow. Strain 2 needs methionine added to the medium to grow. Experiment 1: After strain 1 and strain 2 are mixed in a culture flask containing a minimal medium (containing no added amino acids) and allowed to grow overnight. Results: It is possible isolate new strains that grow on the minimal medium. Experiment 2: Strains...
QUESTION 8 The following situations (1-4) involve different types of gene regulation in prokaryotic cells as shown. OFF...
QUESTION 8 The following situations (1-4) involve different types of gene regulation in prokaryotic cells as shown. OFF and ON reter to whether the gene is transcribed or not. Draw clearly-labelled regulatory proteins and effector molecules in each diagram to explain how the regulation works in each case. The first one has been done for you as an example a) (6 marks) Type of Regulation Regulatory protein effector molecule 1 DNA X negative inducible OFF ON 2 negative repressible DNA...
Note whether in the following strains of E. coli, B-galactosidase will be: (A) Abundant in both...
Note whether in the following strains of E. coli,
B-galactosidase will be:
(A) Abundant in both the presence and absence of lactose
(B) Abundant only in the absence of lactose
(C) Abundant only in the presence of lactose
(D) Abundant only in the presence of sucrose
(D) Absent in both the presence and absence of lactose
Can you answer and explain these please?
What is the correct gene order and which 2 genes are most closely linked? (4 Points) o+at...
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene....
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene. The production of B-galactosidase (+ present or - absent) is measured when lactose is present and absent from the medium. Assume the mutations involve only 1, 0, or Z. A merodiploid is constructed for each of the three strains. The plasmid carries a wild type lac operon and lacl gene. The production of functional B-galactosidase (+ present or - absent) is measured when lactose...
6. A bacterial geneticist mixes 109 cells of E. coli strain A which requires lysine and...
6. A bacterial geneticist mixes 109 cells of E. coli strain A which requires lysine and is ampicillin sensitive with 109 cells of strain B which requires phenylalanine and tyrosine and is ampicillin resistant. In order to detect whether genetic recombination had occurred between the two strains it would be best to grow the mixture of cells in A) minimal glucose medium plus ampicillin B) minimal glucose medium with no supplements C) minimal glucose medium plus lysine and phenylalanine D)...
(c) An E. coli strain was created by transforming with the two plasmids shown below: pm...
(c) An E. coli strain was created by transforming with the two plasmids shown below: pm Tet' gene pxyls HOC 5 G. E. coli Amp' gene Kan gene Mutants of this transformed E. coli strain can be obtained by exposing the cells to dimethyl sulfate. Briefly, explain how you might screen these mutants to obtain an xyls variant that can bind to 4-ethylbenzoic acid 1, carefully explaining the logic underlying your protocol. You can assume that wild type xyls cannot...
Two experiments were performed in order to confirm the Beta-glucuronidase gene from E. coli was present...
Two experiments were performed in order to confirm the
Beta-glucuronidase gene from E. coli was present in the pET28a
plasmid. (Lane1) PCR was performed to amplify the
Beta-glucuronidase gene and if the gene was present then a single
band would appear at ~7,300 bp. However, the band appeared to drag
down the gel but stopped ~7,300 bp. What would cause the PCR
product to smear down the gel and does this mean that the
amplification of the PCR product was...
please answer question 4a-c? What is the recombination frequency between trp and cysX? E) What is...
please answer question 4a-c?
What is the recombination frequency between trp and cysX? E) What is the recombination frequency between ton and cysX? F) What is the order of the three genes? Indicate the distance between them. 4. (3) Suppose that you mate a lac+ Hfr strain with the genotype str 1+ O+ 2+ y + (that you grew in the absence of lactose) with a lac-F-strain with the genotype str - O+ Z-y! You allow sufficient time for the...
3. In a series of mapping experiments, the recombination frequencies for four different linked genes of...
3. In a series of mapping experiments, the recombination frequencies for four different linked genes of Drosophila were determined as shown in the figure below. Wildtype (dominant) is flat wings, medium leg length, brown abdomen, and medium bristle length. w curled wings 1 short legs a - red abdomen b long bristles g23 O a 12 11 0 b 20 3 8 0 w a b The numbers in the boxes are the recombinatior frequencies in between the genes (in...
recombination in phages you have isolated two mutations that affect plaque morphology in phages (y and...
recombination in phages
you have isolated two mutations that affect plaque
morphology in phages (y and z). you conduct a genetic cross between
the two phage strains by coinfecting bacteria with a wild-type
strain and a strain that carries both mutations. phage chromosomes
enter the host and replicate. you collect some of the progeny
phages and plate them onto a lawn of bacterial cells. you observe
the following numbers of plaques:
1. which are examples of recombinant progeny?
Select all...
The following set of three separate experiments was conducted. Two strains of Escherichia coli are used. Strain 1 needs histidine added to the medium to grow. Strain 2 needs methionine added to the medium to grow. Experiment 1: After strain 1 and strain 2 are mixed in a culture flask containing a minimal medium (containing no added amino acids) and allowed to grow overnight. Results: It is possible isolate new strains that grow on the minimal medium. Experiment 2: Strains...
QUESTION 8 The following situations (1-4) involve different types of gene regulation in prokaryotic cells as shown. OFF and ON reter to whether the gene is transcribed or not. Draw clearly-labelled regulatory proteins and effector molecules in each diagram to explain how the regulation works in each case. The first one has been done for you as an example a) (6 marks) Type of Regulation Regulatory protein effector molecule 1 DNA X negative inducible OFF ON 2 negative repressible DNA...
Note whether in the following strains of E. coli,
B-galactosidase will be:
(A) Abundant in both the presence and absence of lactose
(B) Abundant only in the absence of lactose
(C) Abundant only in the presence of lactose
(D) Abundant only in the presence of sucrose
(D) Absent in both the presence and absence of lactose
Can you answer and explain these please?
What is the correct gene order and which 2 genes are most closely linked? (4 Points) o+at...
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene. The production of B-galactosidase (+ present or - absent) is measured when lactose is present and absent from the medium. Assume the mutations involve only 1, 0, or Z. A merodiploid is constructed for each of the three strains. The plasmid carries a wild type lac operon and lacl gene. The production of functional B-galactosidase (+ present or - absent) is measured when lactose...
(c) An E. coli strain was created by transforming with the two plasmids shown below: pm Tet' gene pxyls HOC 5 G. E. coli Amp' gene Kan gene Mutants of this transformed E. coli strain can be obtained by exposing the cells to dimethyl sulfate. Briefly, explain how you might screen these mutants to obtain an xyls variant that can bind to 4-ethylbenzoic acid 1, carefully explaining the logic underlying your protocol. You can assume that wild type xyls cannot...
Two experiments were performed in order to confirm the
Beta-glucuronidase gene from E. coli was present in the pET28a
plasmid. (Lane1) PCR was performed to amplify the
Beta-glucuronidase gene and if the gene was present then a single
band would appear at ~7,300 bp. However, the band appeared to drag
down the gel but stopped ~7,300 bp. What would cause the PCR
product to smear down the gel and does this mean that the
amplification of the PCR product was...
please answer question 4a-c?
What is the recombination frequency between trp and cysX? E) What is the recombination frequency between ton and cysX? F) What is the order of the three genes? Indicate the distance between them. 4. (3) Suppose that you mate a lac+ Hfr strain with the genotype str 1+ O+ 2+ y + (that you grew in the absence of lactose) with a lac-F-strain with the genotype str - O+ Z-y! You allow sufficient time for the...
3. In a series of mapping experiments, the recombination frequencies for four different linked genes of Drosophila were determined as shown in the figure below. Wildtype (dominant) is flat wings, medium leg length, brown abdomen, and medium bristle length. w curled wings 1 short legs a - red abdomen b long bristles g23 O a 12 11 0 b 20 3 8 0 w a b The numbers in the boxes are the recombinatior frequencies in between the genes (in...
recombination in phages
you have isolated two mutations that affect plaque
morphology in phages (y and z). you conduct a genetic cross between
the two phage strains by coinfecting bacteria with a wild-type
strain and a strain that carries both mutations. phage chromosomes
enter the host and replicate. you collect some of the progeny
phages and plate them onto a lawn of bacterial cells. you observe
the following numbers of plaques:
1. which are examples of recombinant progeny?
Select all...
Most questions answered within 3 hours.
-
An empty test tube weighs 15.923 grams. Then,
MgCl2•6H2O is added into the test tube. After...
asked 44 minutes ago -
Please answer true or false. Words
cannot be changed or added in to make it true...
asked 42 minutes ago -
(a) A piston at 6.1 atm contains a gas that occupies a volume of
3.5 L....
asked 43 minutes ago -
Assume memory access is 10 units of time and disk access is
10000 units of time....
asked 1 hour ago -
1. Are all good samples random?
2. Magazines often report surveys giving statistics such as “63%...
asked 1 hour ago -
Under all the various types of market structures, firms
must eventually earn some economic profits for...
asked 1 hour ago -
Consider the following fitness regime for a single locus trait
with two co-dominant alleles: w11 =...
asked 1 hour ago -
A large cable company reports the following.
80% of its customers subscribe to its cable TV...
asked 1 hour ago -
Please answer the question in brief.
Discuss the role of ERP in organizations. Are ERP tools...
asked 1 hour ago -
Discuss the pros and cons of collaborative software such
as SameTime. Does it increase productivity? What...
asked 1 hour ago -
Buying your in-laws a gift because it’s expected is
due to the ____________ motive of gift-giving....
asked 1 hour ago -
Calculate the expected value, the variance, and the standard
deviation of the given random variable X....
asked 2 hours ago