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please answer question 4a-c?
What is the recombination frequency between trp and cysX? E) What is...
4. (3) Suppose that you mate a lac+ Hfr strain with the genotype str S i+...
4. (3) Suppose that you mate a lac+ Hfr strain with the genotype str S i+ o+ z+ y+ (that you grew in the absence of lactose) with a lac - F - strain with the genotype str R i- o+ z - y. You allow sufficient time for the transfer of the genes indicated, with the exception of str S , and plate the bacteria on minimal medium supplemented with arabinose and streptomycin but no lactose. You observe transient...
here are the original strains: please answer C, D, E, and F with full solutions, thank...
here are the original strains:
please answer C, D, E, and F with full solutions, thank you!
:)
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene...
3. (7) Suppose that you have selected E. coli that can grow only when the medium...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...
3. (10 pts) A friend sets up the following cross between an Hfr strain and an...
3. (10 pts) A friend sets up the following cross between an Hfr strain and an F- strain; Hfr: rif lac str pheA X F:rif lac str pheA In the Hfr, the F factor is integrated at 9 minutes on the E. coli genetic map and transfers the genome in a counter clockwise direction, similar to CSHI19 Gene function Position on genetic map Gene Name RNA polymerase (target of rifamycin) lactose utilization phenylalanine biosynthesis ribosomal protein (target of streptomycin) rpoB...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented with arginine, methionine and the antibiotic tetracycline but lacking the essential amino acid isoleucine 3 How would you determine if your recombinant cells had also acquired the tryptophan gene from the Hart Complete the following table indicating which of the organisms (F and/or Hfr used in this experiment would be expected to grow on the given media. Unless specified, MMD is just minimal medium...
1. The type of genetic exchange between bacterial cells that can happen between isolated DNA and...
1. The type of genetic exchange between bacterial cells that can happen between isolated DNA and a live bacterial cell is: a. conjugation b. transduction c. transversion d. transformation 2. An Hfr strain of E. coli with genotype a+b+c+d+e+f+ is mated with an F¯ auxotrophic strain with the genotype a¯b¯c¯d¯e¯f¯. Conjugation is stopped at 10 minute intervals and the genotypes of the resulting conjugants are determined. The following results are obtained: after 10 minutes e+ after 20 minutes a+ e+...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the follow...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the following mutations: lacZ, lacY, laď·0; and lach. For each of these six strains, describe the phenotype you would observe using the following assays. Explain your answers. [Notes: (1) IPTG is a colorless synthetic molecule that acts as an inducer of lac operon expression but cannot serve as a carbon source for bacterial growth because it...
4. Given the following data, what is the recombination frequency between these genes for squash color...
4. Given the following data, what is the recombination frequency between these genes for squash color (red, yellow) and squash shape (log, oval)? What is the distance between the two genes in centiMorgans (or map units)? For full credit, you must show your work, i.e. how you made your calculations. (4 points) Phenotype Progeny from test cross 44 Red, long Yellow, oval Red, oval Yellow, long Total 100
Given the following data, what is the recombination frequency between these genes for squash color (red,...
Given the following data, what is the recombination frequency between these genes for squash color (red, yellow) and squash shape (log, oval)? What is the distance between the two genes in centiMorgans (or map units)? For full credit, you must show your work, i.e. how you made your calculations. (4 points) 3. Answer this question based on the following information. For full credit, you must clearly show how or explain how you came to this answer. Fur color in Labrador...
here are the original strains:
please answer C, D, E, and F with full solutions, thank you!
:)
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...
3. (10 pts) A friend sets up the following cross between an Hfr strain and an F- strain; Hfr: rif lac str pheA X F:rif lac str pheA In the Hfr, the F factor is integrated at 9 minutes on the E. coli genetic map and transfers the genome in a counter clockwise direction, similar to CSHI19 Gene function Position on genetic map Gene Name RNA polymerase (target of rifamycin) lactose utilization phenylalanine biosynthesis ribosomal protein (target of streptomycin) rpoB...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented with arginine, methionine and the antibiotic tetracycline but lacking the essential amino acid isoleucine 3 How would you determine if your recombinant cells had also acquired the tryptophan gene from the Hart Complete the following table indicating which of the organisms (F and/or Hfr used in this experiment would be expected to grow on the given media. Unless specified, MMD is just minimal medium...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the following mutations: lacZ, lacY, laď·0; and lach. For each of these six strains, describe the phenotype you would observe using the following assays. Explain your answers. [Notes: (1) IPTG is a colorless synthetic molecule that acts as an inducer of lac operon expression but cannot serve as a carbon source for bacterial growth because it...
4. Given the following data, what is the recombination frequency between these genes for squash color (red, yellow) and squash shape (log, oval)? What is the distance between the two genes in centiMorgans (or map units)? For full credit, you must show your work, i.e. how you made your calculations. (4 points) Phenotype Progeny from test cross 44 Red, long Yellow, oval Red, oval Yellow, long Total 100
Given the following data, what is the recombination frequency between these genes for squash color (red, yellow) and squash shape (log, oval)? What is the distance between the two genes in centiMorgans (or map units)? For full credit, you must show your work, i.e. how you made your calculations. (4 points) 3. Answer this question based on the following information. For full credit, you must clearly show how or explain how you came to this answer. Fur color in Labrador...
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