Question

C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene in the Hfr strain. Be sure to label distances between markers.

here are the original strains:

please answer C, D, E, and F with full solutions, thank you! :)

Answer 1

3 Arrange according to increasing cyst - 0 min Laet - 9.5 min entry in min. cistomis 9.5min Purt 14.4 to this theet galt - lamin Trypt trist = 38.5 a 2.5min cys / trypt 28 smin some galt Pid D. P. phage : cysxr ton Retrpit Eicoli cysxt togstre- steanbination frequency top & cysx = (10+10 1 a to tw tiged) > boog x 189 % - 10% 6 recombination frequessy behoems tos Recysx : - 10 & X 8% = 5% (H) recombinatim poequency betwers or stop tot (2000 so gese order. So, ton R57 2 5 % тр і ноче х + 10% RIB! top ton cyst is gene order

Hope it's clear..thanks