Homework Answers
The
map of the Ecoli Hfr mutant will be like this
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2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine...
original strains: C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton...
original strains:
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene in the Hfr strain. Be sure to label distances between markers. You have two strains of...
3. (7) Suppose that you have selected E. coli that can grow only when the medium...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
here are the original strains: please answer C, D, E, and F with full solutions, thank...
here are the original strains:
please answer C, D, E, and F with full solutions, thank you!
:)
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...
7. Five Hfr strains A through E are derived from a single F strain of E....
7. Five Hfr strains A through E are derived from a single F strain of E. coli. The following chart shows the entry times in minutes of the first five markers into an F strain when each Hfr strain is used in an interrupted-conjugation experiment: A B C E pro gal mal strs ser ade his ade This gal pro mer 11 16 36 51 13 28 38 44 70 prot met xyl mal" strs 29 32 37 his 10...
This is a Hfr mapping question and I'm completely lost here Basically the bonus is a...
This is a Hfr mapping question and I'm completely lost
here
Basically the bonus is a puzzle. It is based on mapping done with 4 different Hfr strains. I've given you the minutes that that it takes for several of the markers on the map to transfer in as determined by Hfr mapping. All you have to do is put them in the correct order around the circular chromosome and show me how many minutes are in between each marker...
Genetics Question: I am confused on the second part of the question- determining the information for...
Genetics Question: I am confused on the second part of the
question- determining the information for both the donor and
recipient. Thank you!
different interrupted mating experiments, you obtain the following data for 2. Ih three the time of entry of the following genes: arg, met, leu, ser, gal, val, and phe. All of these experiments were conducted using Hfr strains (Hfr 1, Hfr 2, and Hfr3) derived from the same original strain of E. coli. Experiment 1 (Hfr 1)...
Please explain step by step... thank you! In order to determine the overall chromosome map for...
Please explain step by step... thank you!
In order to determine the overall chromosome map for E coli, three Hfr strains were obtained from a protrophic but erythromycin resistant F+ ancestor. The start times of transfer during conjugation matings with F- strains carrying auxotrophic, fermentation, and erythromycin- sensitivity markers were determined for each Hfr strain, as listed. A. Using the times for transfer of each marker, draw a unified chromosome map of E coli, showing the order and relative distances...
2. You have performed the following mating experiment using Hfr and F"strains of Escherichia coli:(4 marks)...
2. You have performed the following mating experiment using Hfr and F"strains of Escherichia coli:(4 marks) Hfr (asp+ trp+ mal+ StrepS) x F (asp-trp-mal- Strep). Strep = streptomycin, an antibiotic Asp = aspartic acid, an amino acid Trp = tryptophan, an amino acid Mal = maltose, a sugar molecule, can be used by some organisms as an energy source rather than glucose (a) What selective media would you use to score recombinant colonies that have received all of the markers...
As a student project, you have isolated six new mutant strains of E. coli with altered...
As a student project, you have isolated six new mutant strains
of E. coli with altered behavior of the lactose operon. The strains
are listed in the table below, together with their phenotypes (with
regard to significant ?-galactosidase synthesis) in three specific
situations.
Columns 1 and 2 present the phenotypes of each mutant haploid
strain. In column 1, the mutant is in an otherwise wild-type
genome. In column 2, the genome also carries a nonsense-suppressor
mutation (that is not present...
original strains:
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene in the Hfr strain. Be sure to label distances between markers. You have two strains of...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
here are the original strains:
please answer C, D, E, and F with full solutions, thank you!
:)
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...
7. Five Hfr strains A through E are derived from a single F strain of E. coli. The following chart shows the entry times in minutes of the first five markers into an F strain when each Hfr strain is used in an interrupted-conjugation experiment: A B C E pro gal mal strs ser ade his ade This gal pro mer 11 16 36 51 13 28 38 44 70 prot met xyl mal" strs 29 32 37 his 10...
This is a Hfr mapping question and I'm completely lost
here
Basically the bonus is a puzzle. It is based on mapping done with 4 different Hfr strains. I've given you the minutes that that it takes for several of the markers on the map to transfer in as determined by Hfr mapping. All you have to do is put them in the correct order around the circular chromosome and show me how many minutes are in between each marker...
Genetics Question: I am confused on the second part of the
question- determining the information for both the donor and
recipient. Thank you!
different interrupted mating experiments, you obtain the following data for 2. Ih three the time of entry of the following genes: arg, met, leu, ser, gal, val, and phe. All of these experiments were conducted using Hfr strains (Hfr 1, Hfr 2, and Hfr3) derived from the same original strain of E. coli. Experiment 1 (Hfr 1)...
Please explain step by step... thank you!
In order to determine the overall chromosome map for E coli, three Hfr strains were obtained from a protrophic but erythromycin resistant F+ ancestor. The start times of transfer during conjugation matings with F- strains carrying auxotrophic, fermentation, and erythromycin- sensitivity markers were determined for each Hfr strain, as listed. A. Using the times for transfer of each marker, draw a unified chromosome map of E coli, showing the order and relative distances...
2. You have performed the following mating experiment using Hfr and F"strains of Escherichia coli:(4 marks) Hfr (asp+ trp+ mal+ StrepS) x F (asp-trp-mal- Strep). Strep = streptomycin, an antibiotic Asp = aspartic acid, an amino acid Trp = tryptophan, an amino acid Mal = maltose, a sugar molecule, can be used by some organisms as an energy source rather than glucose (a) What selective media would you use to score recombinant colonies that have received all of the markers...
As a student project, you have isolated six new mutant strains
of E. coli with altered behavior of the lactose operon. The strains
are listed in the table below, together with their phenotypes (with
regard to significant ?-galactosidase synthesis) in three specific
situations.
Columns 1 and 2 present the phenotypes of each mutant haploid
strain. In column 1, the mutant is in an otherwise wild-type
genome. In column 2, the genome also carries a nonsense-suppressor
mutation (that is not present...
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