Homework Answers
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7. Five Hfr strains A through E are derived from a single F strain of E....
original strains: C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton...
original strains:
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene in the Hfr strain. Be sure to label distances between markers. You have two strains of...
You use à new strain of Hfr E. coli to map several genes by using interrupted...
You use à new strain of Hfr E. coli to map several genes by using interrupted conjugation. You mix Hfr cells that are xy/+ mil+ mal+ met+ and sensitive to phage T6. You mix those cells with an F- strain that is xyl- mil- mal- met- and resistant to phage T6 to allow conjugation. At regular intervals, you remove samples and interrupt conjugation. You kill the Hfr cells in each sample by using the T6 phage and plate what's left...
here are the original strains: please answer C, D, E, and F with full solutions, thank...
here are the original strains:
please answer C, D, E, and F with full solutions, thank you!
:)
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene...
2. You have performed the following mating experiment using Hfr and F"strains of Escherichia coli:(4 marks)...
2. You have performed the following mating experiment using Hfr and F"strains of Escherichia coli:(4 marks) Hfr (asp+ trp+ mal+ StrepS) x F (asp-trp-mal- Strep). Strep = streptomycin, an antibiotic Asp = aspartic acid, an amino acid Trp = tryptophan, an amino acid Mal = maltose, a sugar molecule, can be used by some organisms as an energy source rather than glucose (a) What selective media would you use to score recombinant colonies that have received all of the markers...
Four Hfr strains (1, 2, 3, 4) were isolated from the same prototrophic, strF strain. In...
Four Hfr strains (1, 2, 3, 4) were isolated from the same prototrophic, strF strain. In separate experiments using the technique of interrupted mating, these four Hfr strains donated the following markers to an auxotrophic, str F strain in the order shown: (only the first six genes transmitted were scored for each strain) Hfr Strain 1 Hfr Strain 2 Hfr Strain 3 Hfr Strain 4 A. What is the order of these markers on the circular chromosome of the original...
2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine...
2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine the location of the his mutation on the E. coli chromosome, you perform interrupted mating experiments with 5 different Hfr strains. The following chart shows the time of entry (minutes, in parentheses) of the wild-type alleles of the first 5 markers (mutant genes) into the His strain. + + Hfr A. Hfr B Hfr CH Hfr D. Hfr E- his (3) cys (11)- arg...
33. In E. coli, a streptomycin-sensitive, prototrophic Hfr strain was mated to a streptomycin-resistant F-strain auxotrophic...
33. In E. coli, a streptomycin-sensitive, prototrophic Hfr strain was mated to a streptomycin-resistant F-strain auxotrophic for a number of nutritional requirements. After conjugation for various times, the results shown at right were obtained. Of the four genes shown, which two genes map the closest to each other? azir tonr Percent of recipients receiving a given gene lact gal + 10 50 60 20 30 40 Time (minutes)
This is a Hfr mapping question and I'm completely lost here Basically the bonus is a...
This is a Hfr mapping question and I'm completely lost
here
Basically the bonus is a puzzle. It is based on mapping done with 4 different Hfr strains. I've given you the minutes that that it takes for several of the markers on the map to transfer in as determined by Hfr mapping. All you have to do is put them in the correct order around the circular chromosome and show me how many minutes are in between each marker...
You perform interrupted conjugation using an a+b+c+d+e+f+g+h+ Hfr strain and an F– strain that is a–b–c–d–e–f–g–h–....
You perform interrupted conjugation using an a+b+c+d+e+f+g+h+ Hfr strain and an F– strain that is a–b–c–d–e–f–g–h–. You observe the following genes transferred together in order from last to first: h+b+g+d+ e+f+d+g+ f+e+a+c+ What is the map order of the genes?
An Hfr strain of E. coli with genotype a+b+c+d+e+f+ is mated with an F¯ auxotrophic strain...
An Hfr strain of E. coli with genotype a+b+c+d+e+f+ is mated with an F¯ auxotrophic strain with the genotype a¯b¯c¯d¯e¯f¯. Conjugation is stopped at 10 minute intervals and the genotypes of the resulting conjugants are determined. The following results are obtained: after 10 minutes e+ after 20 minutes a+ e+ after 30 minutes a+ b+e+ after 40 minutes a+b+d+e+ after 50 minutes a+b+c+d+e+ after 60 minutes a+b+c+d+e+f+ Which represents the correct order of genes on this bacterial chromosomes? efabcd eabdcf...
original strains:
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene in the Hfr strain. Be sure to label distances between markers. You have two strains of...
You use à new strain of Hfr E. coli to map several genes by using interrupted conjugation. You mix Hfr cells that are xy/+ mil+ mal+ met+ and sensitive to phage T6. You mix those cells with an F- strain that is xyl- mil- mal- met- and resistant to phage T6 to allow conjugation. At regular intervals, you remove samples and interrupt conjugation. You kill the Hfr cells in each sample by using the T6 phage and plate what's left...
here are the original strains:
please answer C, D, E, and F with full solutions, thank you!
:)
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene...
2. You have performed the following mating experiment using Hfr and F"strains of Escherichia coli:(4 marks) Hfr (asp+ trp+ mal+ StrepS) x F (asp-trp-mal- Strep). Strep = streptomycin, an antibiotic Asp = aspartic acid, an amino acid Trp = tryptophan, an amino acid Mal = maltose, a sugar molecule, can be used by some organisms as an energy source rather than glucose (a) What selective media would you use to score recombinant colonies that have received all of the markers...
Four Hfr strains (1, 2, 3, 4) were isolated from the same prototrophic, strF strain. In separate experiments using the technique of interrupted mating, these four Hfr strains donated the following markers to an auxotrophic, str F strain in the order shown: (only the first six genes transmitted were scored for each strain) Hfr Strain 1 Hfr Strain 2 Hfr Strain 3 Hfr Strain 4 A. What is the order of these markers on the circular chromosome of the original...
2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine the location of the his mutation on the E. coli chromosome, you perform interrupted mating experiments with 5 different Hfr strains. The following chart shows the time of entry (minutes, in parentheses) of the wild-type alleles of the first 5 markers (mutant genes) into the His strain. + + Hfr A. Hfr B Hfr CH Hfr D. Hfr E- his (3) cys (11)- arg...
33. In E. coli, a streptomycin-sensitive, prototrophic Hfr strain was mated to a streptomycin-resistant F-strain auxotrophic for a number of nutritional requirements. After conjugation for various times, the results shown at right were obtained. Of the four genes shown, which two genes map the closest to each other? azir tonr Percent of recipients receiving a given gene lact gal + 10 50 60 20 30 40 Time (minutes)
This is a Hfr mapping question and I'm completely lost
here
Basically the bonus is a puzzle. It is based on mapping done with 4 different Hfr strains. I've given you the minutes that that it takes for several of the markers on the map to transfer in as determined by Hfr mapping. All you have to do is put them in the correct order around the circular chromosome and show me how many minutes are in between each marker...
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