Homework Answers
azi r and tan r genes are closest to each other beacuse beacuse there is less time difference between the two gene it means they are present very near to each another than other genes.
Add Answer to:
33. In E. coli, a streptomycin-sensitive, prototrophic Hfr strain was mated to a streptomycin-resistant F-strain auxotrophic...
An Hfr strain of E. coli with genotype a+b+c+d+e+f+ is mated with an F¯ auxotrophic strain...
An Hfr strain of E. coli with genotype a+b+c+d+e+f+ is mated with an F¯ auxotrophic strain with the genotype a¯b¯c¯d¯e¯f¯. Conjugation is stopped at 10 minute intervals and the genotypes of the resulting conjugants are determined. The following results are obtained: after 10 minutes e+ after 20 minutes a+ e+ after 30 minutes a+ b+e+ after 40 minutes a+b+d+e+ after 50 minutes a+b+c+d+e+ after 60 minutes a+b+c+d+e+f+ Which represents the correct order of genes on this bacterial chromosomes? efabcd eabdcf...
You use à new strain of Hfr E. coli to map several genes by using interrupted...
You use à new strain of Hfr E. coli to map several genes by using interrupted conjugation. You mix Hfr cells that are xy/+ mil+ mal+ met+ and sensitive to phage T6. You mix those cells with an F- strain that is xyl- mil- mal- met- and resistant to phage T6 to allow conjugation. At regular intervals, you remove samples and interrupt conjugation. You kill the Hfr cells in each sample by using the T6 phage and plate what's left...
A streptomycin-sensitive Hfr strain, prototrophic for threonine (thr), leucine (leu), arginine (arg), and histidine (his), was...
A streptomycin-sensitive Hfr strain, prototrophic for threonine
(thr), leucine (leu), arginine (arg), and histidine (his), was
allowed to conjugate with a streptomycin-resistant F- strain whose
genotype was thr- leu- arg- his-. When plated on complete medium
containing streptomycin, a number of colonies arose. Cells from
five of these colonies were then tested on minimal medium
supplemented with added nutrients as indicated below. What were the
genotypes of the five colonies. PLEASE EXPLAIN.
MINIMAL MEDIUM PLUS COLONIES THR LEU THR HIS...
7. Five Hfr strains A through E are derived from a single F strain of E....
7. Five Hfr strains A through E are derived from a single F strain of E. coli. The following chart shows the entry times in minutes of the first five markers into an F strain when each Hfr strain is used in an interrupted-conjugation experiment: A B C E pro gal mal strs ser ade his ade This gal pro mer 11 16 36 51 13 28 38 44 70 prot met xyl mal" strs 29 32 37 his 10...
Please explain step by step... thank you! In order to determine the overall chromosome map for...
Please explain step by step... thank you!
In order to determine the overall chromosome map for E coli, three Hfr strains were obtained from a protrophic but erythromycin resistant F+ ancestor. The start times of transfer during conjugation matings with F- strains carrying auxotrophic, fermentation, and erythromycin- sensitivity markers were determined for each Hfr strain, as listed. A. Using the times for transfer of each marker, draw a unified chromosome map of E coli, showing the order and relative distances...
3. (10 pts) A friend sets up the following cross between an Hfr strain and an...
3. (10 pts) A friend sets up the following cross between an Hfr strain and an F- strain; Hfr: rif lac str pheA X F:rif lac str pheA In the Hfr, the F factor is integrated at 9 minutes on the E. coli genetic map and transfers the genome in a counter clockwise direction, similar to CSHI19 Gene function Position on genetic map Gene Name RNA polymerase (target of rifamycin) lactose utilization phenylalanine biosynthesis ribosomal protein (target of streptomycin) rpoB...
2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine...
2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine the location of the his mutation on the E. coli chromosome, you perform interrupted mating experiments with 5 different Hfr strains. The following chart shows the time of entry (minutes, in parentheses) of the wild-type alleles of the first 5 markers (mutant genes) into the His strain. + + Hfr A. Hfr B Hfr CH Hfr D. Hfr E- his (3) cys (11)- arg...
Question 10 View Policies Current Attempt In Progress In a conjugation experiment between two strains of...
Question 10 View Policies Current Attempt In Progress In a conjugation experiment between two strains of E.coli (streptomycin-sensitive Hfr strain and a streptomycin-resistant F-straint, aliquots were extracted from the mating mixture at various time points and plated on minimal media supplemented with streptomycin and a variety of amino acids. The number of conjugates at each time point on each type of media are shown in the table below. What can you conclude from these data!! Time (minutes) Medium MM (-)...
This is a Hfr mapping question and I'm completely lost here Basically the bonus is a...
This is a Hfr mapping question and I'm completely lost
here
Basically the bonus is a puzzle. It is based on mapping done with 4 different Hfr strains. I've given you the minutes that that it takes for several of the markers on the map to transfer in as determined by Hfr mapping. All you have to do is put them in the correct order around the circular chromosome and show me how many minutes are in between each marker...
1. The type of genetic exchange between bacterial cells that can happen between isolated DNA and...
1. The type of genetic exchange between bacterial cells that can happen between isolated DNA and a live bacterial cell is: a. conjugation b. transduction c. transversion d. transformation 2. An Hfr strain of E. coli with genotype a+b+c+d+e+f+ is mated with an F¯ auxotrophic strain with the genotype a¯b¯c¯d¯e¯f¯. Conjugation is stopped at 10 minute intervals and the genotypes of the resulting conjugants are determined. The following results are obtained: after 10 minutes e+ after 20 minutes a+ e+...
You use à new strain of Hfr E. coli to map several genes by using interrupted conjugation. You mix Hfr cells that are xy/+ mil+ mal+ met+ and sensitive to phage T6. You mix those cells with an F- strain that is xyl- mil- mal- met- and resistant to phage T6 to allow conjugation. At regular intervals, you remove samples and interrupt conjugation. You kill the Hfr cells in each sample by using the T6 phage and plate what's left...
A streptomycin-sensitive Hfr strain, prototrophic for threonine
(thr), leucine (leu), arginine (arg), and histidine (his), was
allowed to conjugate with a streptomycin-resistant F- strain whose
genotype was thr- leu- arg- his-. When plated on complete medium
containing streptomycin, a number of colonies arose. Cells from
five of these colonies were then tested on minimal medium
supplemented with added nutrients as indicated below. What were the
genotypes of the five colonies. PLEASE EXPLAIN.
MINIMAL MEDIUM PLUS COLONIES THR LEU THR HIS...
7. Five Hfr strains A through E are derived from a single F strain of E. coli. The following chart shows the entry times in minutes of the first five markers into an F strain when each Hfr strain is used in an interrupted-conjugation experiment: A B C E pro gal mal strs ser ade his ade This gal pro mer 11 16 36 51 13 28 38 44 70 prot met xyl mal" strs 29 32 37 his 10...
Please explain step by step... thank you!
In order to determine the overall chromosome map for E coli, three Hfr strains were obtained from a protrophic but erythromycin resistant F+ ancestor. The start times of transfer during conjugation matings with F- strains carrying auxotrophic, fermentation, and erythromycin- sensitivity markers were determined for each Hfr strain, as listed. A. Using the times for transfer of each marker, draw a unified chromosome map of E coli, showing the order and relative distances...
3. (10 pts) A friend sets up the following cross between an Hfr strain and an F- strain; Hfr: rif lac str pheA X F:rif lac str pheA In the Hfr, the F factor is integrated at 9 minutes on the E. coli genetic map and transfers the genome in a counter clockwise direction, similar to CSHI19 Gene function Position on genetic map Gene Name RNA polymerase (target of rifamycin) lactose utilization phenylalanine biosynthesis ribosomal protein (target of streptomycin) rpoB...
2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine the location of the his mutation on the E. coli chromosome, you perform interrupted mating experiments with 5 different Hfr strains. The following chart shows the time of entry (minutes, in parentheses) of the wild-type alleles of the first 5 markers (mutant genes) into the His strain. + + Hfr A. Hfr B Hfr CH Hfr D. Hfr E- his (3) cys (11)- arg...
Question 10 View Policies Current Attempt In Progress In a conjugation experiment between two strains of E.coli (streptomycin-sensitive Hfr strain and a streptomycin-resistant F-straint, aliquots were extracted from the mating mixture at various time points and plated on minimal media supplemented with streptomycin and a variety of amino acids. The number of conjugates at each time point on each type of media are shown in the table below. What can you conclude from these data!! Time (minutes) Medium MM (-)...
This is a Hfr mapping question and I'm completely lost
here
Basically the bonus is a puzzle. It is based on mapping done with 4 different Hfr strains. I've given you the minutes that that it takes for several of the markers on the map to transfer in as determined by Hfr mapping. All you have to do is put them in the correct order around the circular chromosome and show me how many minutes are in between each marker...
Most questions answered within 3 hours.
-
Write a program to solve the Josephus problem, with the following
modification:
Sample Input:
./a.out n...
asked 1 hour ago -
At the start of a CD it is spinning at a rate of 525 rpm
(revolutions...
asked 1 hour ago -
4. Without doing any calculations, predict whether the observed
∆T would increase, decrease or remain the...
asked 3 hours ago -
Based on the range, which of the following sets of scores has
the greatest variability? 3,...
asked 4 hours ago -
Ripples in a pond travel at a velocity of 3 m/s with one peak
passing a...
asked 4 hours ago -
A man stands on the roof of a building of height 13.0 mm and
throws a...
asked 4 hours ago -
The extent to which assets are financed by borrowed funds and
other liabilities is indicated by:...
asked 5 hours ago -
Explain in detail
Germany is the fifth largest economy
explain what goods and services Germany specializes...
asked 5 hours ago -
The density of platinum is 21.45 g/mL. If a cube of platinum
with a mass of...
asked 5 hours ago -
Accounts Receivable
Sales
A/R Posting
Extended Sales Invoice
Packing Slip
Compare invoice to packing slip 2...
asked 5 hours ago -
Michaella, age 23, is a full-time law student and is claimed by
her parents as a...
asked 5 hours ago -
Why are polymers not typically casted into products?
asked 5 hours ago