A streptomycin-sensitive Hfr strain, prototrophic for threonine (thr), leucine (leu), arginine (arg), and histidine (his), was allowed to conjugate with a streptomycin-resistant F- strain whose genotype was thr- leu- arg- his-. When plated on complete medium containing streptomycin, a number of colonies arose. Cells from five of these colonies were then tested on minimal medium supplemented with added nutrients as indicated below. What were the genotypes of the five colonies. PLEASE EXPLAIN.
Homework Answers
Colony1- thr -leu-arg-his+
Colony 2- thr+ leu+ arg- his-
Colony 3- thr- leu- arg+ his-
Colony 4- thr+ leu- arg- his+
Colony 5- thr+ leu-arg- his-
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A streptomycin-sensitive Hfr strain, prototrophic for threonine
(thr), leucine (leu), arginine (arg), and histidine (his), was...
Please answer all the questions. No explanation needed. 6. (pr) If a met-thr-Str. Hfr strain is...
Please answer all the questions. No explanation needed.
6. (pr) If a met-thr-Str. Hfr strain is mated with an F- of genotype lew-thi-Str prototrophic recombinants may be detected by plating the mixture on: A. Leucine and methionine + Streptomycin B. Threonine and thiamine + Streptomycin C. Leucine and thiamine and Streptomycin D. Minimal Medium + Streptomycin E. Methionine, threonine, leucine, and thiamine + Streptomycin 7. ( ) From an F+ strain with markers edebin that circular order), which of the...
strain (thrleut his pro pan bio). At each gene, + . (5 points) One triply auxotrophic...
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2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented with arginine, methionine and the antibiotic tetracycline but lacking the essential amino acid isoleucine 3 How would you determine if your recombinant cells had also acquired the tryptophan gene from the Hart Complete the following table indicating which of the organisms (F and/or Hfr used in this experiment would be expected to grow on the given media. Unless specified, MMD is just minimal medium...
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Please answer all the questions. No explanation needed.
6. (pr) If a met-thr-Str. Hfr strain is mated with an F- of genotype lew-thi-Str prototrophic recombinants may be detected by plating the mixture on: A. Leucine and methionine + Streptomycin B. Threonine and thiamine + Streptomycin C. Leucine and thiamine and Streptomycin D. Minimal Medium + Streptomycin E. Methionine, threonine, leucine, and thiamine + Streptomycin 7. ( ) From an F+ strain with markers edebin that circular order), which of the...
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