Question

This question will help you predict the outcome of the FOA assay in week 11. Prior knowledge: (1) The genotype of the yeast reporter strain AGY75 is "trp1; ura3; his 3; msh2A; PSH44". It carries the mutated/non-functional alleles for gene Trp1; Ura3; His3; a deletion that removed MSH2; and the PSH44 plasmid. (2) PMSH2 plasmid carries gene AmpR, His 3, MSH2 (3) pSH44 plasmid carries gene GT16.5-Ura3, Trp1 (4) SD/-His, -Trp, -Leu, -Thr, +FOA plate: SD medium containing FOA, and all essential nutrients except for histidine, tryptophan, leucine, and threonine. Determine whether the yeast will grow under the following conditions. ✓ AGY75 transformed with pMSH2, then plated on SD/-His, -Trp, -Leu, -Thr, +FOA media AGY75 transformed with pMSH2' (assume that the mutant MSH2 is nonfunctional), then plated on SD/-His, -Trp, -Leu, -Thr, +FOA media Choose... can not be determined no growth growth Choose... AGY75 transformed with PRS413, then plated on SD/-His, -Trp, -Leu, -Thr, +FOA media AGY75 transformed with pMSH2, then plated on SD/-His, -Trp, -Leu, -Thr, -Ura, +FOA media Choose... AGY75 transformed with pMSH2' (assume that the mutant MSH2 is nonfunctional), then plated on SD/-His, -Trp, -Leu, -Thr, -Ura, +FOA media Choose... AGY75 transformed with PRS413, then plated on SD/-His, -Trp, -Leu, -Thr, -Ura, +FOA media Choose...