Homework Answers
The region 75 Bp to 250 Bp is covered with RNA Polymerase and the transcription factors. Total length covered
is (250 - 75) = 175 Bp.
B. If we added RNA polymerase only then it will cover around 80 Bp. We should observe more bands in lane to between 75 Bp and 250 Bp
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11) A researcher performed a DNase footprinting experiment with a 300 bp eukaryotic DNA, which contained...
32P-labeled promoter DNA was mixed, in various combinations, with the general transcription factors TFIID (D), TFIIA (A...
32P-labeled promoter DNA was mixed, in various combinations, with the general transcription factors TFIID (D), TFIIA (A), TFIIB (B), TFIIF (F), and RNA polymerase II (Pol II). Binding of these factors to the promoter DNA was autoradiograph of the gel is shown below. The anode (+ electrode) is at the bottom. Labels on top of each lane indicate the factors mixed with DNA. For binding reactions 3-7 (lanes 3-7), the concentration of RNA Pol II was continually increased from zero...
A 3.1-kb fragment of DNA containing a complete human gene has been made available to you....
A 3.1-kb fragment of DNA containing a complete human gene has
been made available to you. The DNA segment contains the promoter
sequence and extends beyond the polyadenylation site for the gene.
You decide to do an electromobility shift assay (EMSA) to determine
what molecules bind to the fragment. The results are shown below.
If Lane 1 contains only the 3.1-kb fragment, which answer correctly
describes another lane of the experiment?
a. Lane 2 contains the 3.1kb fragment + TFIIB...
can someone explain how to answer this with reasons why 30-45 mins after estrogen addition: Acchyiase...
can someone explain how to answer this with reasons why
30-45 mins after estrogen addition: Acchyiase (HAT, odde auhye 60-90 mins after estrogen addition: Reauitment ef Poy to open 7. T identify the Gene X DNA element responsible for regulation by a Growth Fagor, you perform a linker scanning experiment in which you mutate 10 bp Tegions centered around positions -205 to-5 in the Gene X promoter/promoter- proximal region (wt wild-type promoter). The promoter variants were tested for their ability...
i need help with 10 and 11 please The extent of condensation of chromatin plays an...
i need help with 10 and 11 please
The extent of condensation of chromatin plays an important role in the regulation of RNA transcription in eukaryotic cells. Chromatin-remodeling complexes are among proteins that control the physical state and functional activity of chromatin. The experiment described shortly was designed to study the role of a chromatin remodeling complex designated RSC (Remodeling the Structure of Chromatin) in the regulation of nucleosome dynamics. Figure 1 shows the results of an in vitro experiment....
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard the supernatant, but keep the pellet. In step 15, you discard the pellet, but keep the supernatant. Explain why the pattern is different between the two steps and the consequence of mixing up these two steps. Procedure Part 1: mt DNA Isolation from your cheek cells. Lysis solution is used to breakdown the cells in this step, you will isolate MEONA from cheek cells....
32P-labeled promoter DNA was mixed, in various combinations, with the general transcription factors TFIID (D), TFIIA (A), TFIIB (B), TFIIF (F), and RNA polymerase II (Pol II). Binding of these factors to the promoter DNA was autoradiograph of the gel is shown below. The anode (+ electrode) is at the bottom. Labels on top of each lane indicate the factors mixed with DNA. For binding reactions 3-7 (lanes 3-7), the concentration of RNA Pol II was continually increased from zero...
A 3.1-kb fragment of DNA containing a complete human gene has
been made available to you. The DNA segment contains the promoter
sequence and extends beyond the polyadenylation site for the gene.
You decide to do an electromobility shift assay (EMSA) to determine
what molecules bind to the fragment. The results are shown below.
If Lane 1 contains only the 3.1-kb fragment, which answer correctly
describes another lane of the experiment?
a. Lane 2 contains the 3.1kb fragment + TFIIB...
can someone explain how to answer this with reasons why
30-45 mins after estrogen addition: Acchyiase (HAT, odde auhye 60-90 mins after estrogen addition: Reauitment ef Poy to open 7. T identify the Gene X DNA element responsible for regulation by a Growth Fagor, you perform a linker scanning experiment in which you mutate 10 bp Tegions centered around positions -205 to-5 in the Gene X promoter/promoter- proximal region (wt wild-type promoter). The promoter variants were tested for their ability...
i need help with 10 and 11 please
The extent of condensation of chromatin plays an important role in the regulation of RNA transcription in eukaryotic cells. Chromatin-remodeling complexes are among proteins that control the physical state and functional activity of chromatin. The experiment described shortly was designed to study the role of a chromatin remodeling complex designated RSC (Remodeling the Structure of Chromatin) in the regulation of nucleosome dynamics. Figure 1 shows the results of an in vitro experiment....
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard the supernatant, but keep the pellet. In step 15, you discard the pellet, but keep the supernatant. Explain why the pattern is different between the two steps and the consequence of mixing up these two steps. Procedure Part 1: mt DNA Isolation from your cheek cells. Lysis solution is used to breakdown the cells in this step, you will isolate MEONA from cheek cells....
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