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Please explain step by step... thank you!
In order to determine the overall chromosome map for...
This is a Hfr mapping question and I'm completely lost here Basically the bonus is a...
This is a Hfr mapping question and I'm completely lost
here
Basically the bonus is a puzzle. It is based on mapping done with 4 different Hfr strains. I've given you the minutes that that it takes for several of the markers on the map to transfer in as determined by Hfr mapping. All you have to do is put them in the correct order around the circular chromosome and show me how many minutes are in between each marker...
2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine...
2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine the location of the his mutation on the E. coli chromosome, you perform interrupted mating experiments with 5 different Hfr strains. The following chart shows the time of entry (minutes, in parentheses) of the wild-type alleles of the first 5 markers (mutant genes) into the His strain. + + Hfr A. Hfr B Hfr CH Hfr D. Hfr E- his (3) cys (11)- arg...
7. Five Hfr strains A through E are derived from a single F strain of E....
7. Five Hfr strains A through E are derived from a single F strain of E. coli. The following chart shows the entry times in minutes of the first five markers into an F strain when each Hfr strain is used in an interrupted-conjugation experiment: A B C E pro gal mal strs ser ade his ade This gal pro mer 11 16 36 51 13 28 38 44 70 prot met xyl mal" strs 29 32 37 his 10...
I am having trouble in understanding this question. The second photo is part b of the question. A...
I am having trouble in understanding this question. The second
photo is part b of the question. Any help would be
appreciated!
Four Hfr strains of E.ole known to transfer thier genetic material during conjugation in different sequences. Given the time of entry of the markers into the F-recipient, construct a genetic map which includes all of these markers, starting with arg as number 1 and phe as number 11.(Not all markers were assayed in each strain, remember the E.coli...
You use à new strain of Hfr E. coli to map several genes by using interrupted...
You use à new strain of Hfr E. coli to map several genes by using interrupted conjugation. You mix Hfr cells that are xy/+ mil+ mal+ met+ and sensitive to phage T6. You mix those cells with an F- strain that is xyl- mil- mal- met- and resistant to phage T6 to allow conjugation. At regular intervals, you remove samples and interrupt conjugation. You kill the Hfr cells in each sample by using the T6 phage and plate what's left...
here are the original strains: please answer C, D, E, and F with full solutions, thank...
here are the original strains:
please answer C, D, E, and F with full solutions, thank you!
:)
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene...
Genetics Question: I am confused on the second part of the question- determining the information for...
Genetics Question: I am confused on the second part of the
question- determining the information for both the donor and
recipient. Thank you!
different interrupted mating experiments, you obtain the following data for 2. Ih three the time of entry of the following genes: arg, met, leu, ser, gal, val, and phe. All of these experiments were conducted using Hfr strains (Hfr 1, Hfr 2, and Hfr3) derived from the same original strain of E. coli. Experiment 1 (Hfr 1)...
Sam is working in a genetics lab this summer in order to gain more hands-on experience...
Sam is working in a genetics lab this summer in order to gain more hands-on experience before he applies to graduate school. The professor with whom he is working is particularly interested in studying a new strain of E.coli and assigns Sam an interrupted mating experiment to perform. Sam is attempting to determine the order of 7 different genes on the bacterial chromosome: Ger, Fra, Lod, Moc, Bla, Jat, and Arp. Sam used four different Hfr strains (Hfr 1, Hfr2,...
3. (7) Suppose that you have selected E. coli that can grow only when the medium...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
genetics questions. Please help thank you! t0 1-3. Match each of the following with the correct...
genetics questions. Please help thank you!
t0 1-3. Match each of the following with the correct type of gene transfer in bacteria (conjugation, transformation, or transduction) niee (a) Hfr x F- (b) Bacteriophage needed (c) Naked DNA transferred (d) Plaques formed (e) Direct contact between bacterial cells () F+xF- In Drosophild, the ganes ct (cut wing).yello ly), and v (vermilion cye color) arc all X-linked. Females hetcrozygous for all thrcc markers were mated with wildtype males, and the following malc...
This is a Hfr mapping question and I'm completely lost
here
Basically the bonus is a puzzle. It is based on mapping done with 4 different Hfr strains. I've given you the minutes that that it takes for several of the markers on the map to transfer in as determined by Hfr mapping. All you have to do is put them in the correct order around the circular chromosome and show me how many minutes are in between each marker...
2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine the location of the his mutation on the E. coli chromosome, you perform interrupted mating experiments with 5 different Hfr strains. The following chart shows the time of entry (minutes, in parentheses) of the wild-type alleles of the first 5 markers (mutant genes) into the His strain. + + Hfr A. Hfr B Hfr CH Hfr D. Hfr E- his (3) cys (11)- arg...
7. Five Hfr strains A through E are derived from a single F strain of E. coli. The following chart shows the entry times in minutes of the first five markers into an F strain when each Hfr strain is used in an interrupted-conjugation experiment: A B C E pro gal mal strs ser ade his ade This gal pro mer 11 16 36 51 13 28 38 44 70 prot met xyl mal" strs 29 32 37 his 10...
I am having trouble in understanding this question. The second
photo is part b of the question. Any help would be
appreciated!
Four Hfr strains of E.ole known to transfer thier genetic material during conjugation in different sequences. Given the time of entry of the markers into the F-recipient, construct a genetic map which includes all of these markers, starting with arg as number 1 and phe as number 11.(Not all markers were assayed in each strain, remember the E.coli...
You use à new strain of Hfr E. coli to map several genes by using interrupted conjugation. You mix Hfr cells that are xy/+ mil+ mal+ met+ and sensitive to phage T6. You mix those cells with an F- strain that is xyl- mil- mal- met- and resistant to phage T6 to allow conjugation. At regular intervals, you remove samples and interrupt conjugation. You kill the Hfr cells in each sample by using the T6 phage and plate what's left...
here are the original strains:
please answer C, D, E, and F with full solutions, thank you!
:)
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene...
Genetics Question: I am confused on the second part of the
question- determining the information for both the donor and
recipient. Thank you!
different interrupted mating experiments, you obtain the following data for 2. Ih three the time of entry of the following genes: arg, met, leu, ser, gal, val, and phe. All of these experiments were conducted using Hfr strains (Hfr 1, Hfr 2, and Hfr3) derived from the same original strain of E. coli. Experiment 1 (Hfr 1)...
Sam is working in a genetics lab this summer in order to gain more hands-on experience before he applies to graduate school. The professor with whom he is working is particularly interested in studying a new strain of E.coli and assigns Sam an interrupted mating experiment to perform. Sam is attempting to determine the order of 7 different genes on the bacterial chromosome: Ger, Fra, Lod, Moc, Bla, Jat, and Arp. Sam used four different Hfr strains (Hfr 1, Hfr2,...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
genetics questions. Please help thank you!
t0 1-3. Match each of the following with the correct type of gene transfer in bacteria (conjugation, transformation, or transduction) niee (a) Hfr x F- (b) Bacteriophage needed (c) Naked DNA transferred (d) Plaques formed (e) Direct contact between bacterial cells () F+xF- In Drosophild, the ganes ct (cut wing).yello ly), and v (vermilion cye color) arc all X-linked. Females hetcrozygous for all thrcc markers were mated with wildtype males, and the following malc...
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