Homework Answers
Ans) I have attached the diagram representing the order of the markers along with the distance (in minutes) between the each transferred marker when conjugation occurred between F and Hfr strain.
You can observe that cys+ was already present in the F strain (0 minutes), after 9.5 minutes lac+ was transferred, 5 minutes after lac+, pur+ was transferred ( i.e 9.5 + 5 = 14.5 minutes) and so on.Likewise you have to calculate.
However,the bacterial chromosome is always circular
( The first diagram was just for better understanding and to avoid confusion) so the final result of the conjugation will be as follows :
Add Answer to:
original strains:
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton...
here are the original strains: please answer C, D, E, and F with full solutions, thank...
here are the original strains:
please answer C, D, E, and F with full solutions, thank you!
:)
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene...
3. (7) Suppose that you have selected E. coli that can grow only when the medium...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...
2. You have performed the following mating experiment using Hfr and F"strains of Escherichia coli:(4 marks)...
2. You have performed the following mating experiment using Hfr and F"strains of Escherichia coli:(4 marks) Hfr (asp+ trp+ mal+ StrepS) x F (asp-trp-mal- Strep). Strep = streptomycin, an antibiotic Asp = aspartic acid, an amino acid Trp = tryptophan, an amino acid Mal = maltose, a sugar molecule, can be used by some organisms as an energy source rather than glucose (a) What selective media would you use to score recombinant colonies that have received all of the markers...
You are performing an interrupted mating experiment testing for transfer of lac, gal, strR, ile, trp,...
You are performing an interrupted mating experiment testing for transfer of lac, gal, strR, ile, trp, tonR, and val. You have the following plates available to you: Plate A: MM Plate B: MM + lac + val + ile + trp Plate C: MM + gal + T1 phage Plate D: MM + val Plate E: MM + streptomycin + ile + trp Plate F: MM + lac + trp Which of the above plates selects for transfer of the...
2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine...
2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine the location of the his mutation on the E. coli chromosome, you perform interrupted mating experiments with 5 different Hfr strains. The following chart shows the time of entry (minutes, in parentheses) of the wild-type alleles of the first 5 markers (mutant genes) into the His strain. + + Hfr A. Hfr B Hfr CH Hfr D. Hfr E- his (3) cys (11)- arg...
7. Five Hfr strains A through E are derived from a single F strain of E....
7. Five Hfr strains A through E are derived from a single F strain of E. coli. The following chart shows the entry times in minutes of the first five markers into an F strain when each Hfr strain is used in an interrupted-conjugation experiment: A B C E pro gal mal strs ser ade his ade This gal pro mer 11 16 36 51 13 28 38 44 70 prot met xyl mal" strs 29 32 37 his 10...
This is a Hfr mapping question and I'm completely lost here Basically the bonus is a...
This is a Hfr mapping question and I'm completely lost
here
Basically the bonus is a puzzle. It is based on mapping done with 4 different Hfr strains. I've given you the minutes that that it takes for several of the markers on the map to transfer in as determined by Hfr mapping. All you have to do is put them in the correct order around the circular chromosome and show me how many minutes are in between each marker...
1. The following diagram shows the genetic map for a chromosome. A B C D 10cM...
1. The following diagram shows the genetic map for a chromosome. A B C D 10cM 5cM 40cM If a diploid female heterozygous for A and D (A D//a d) is testcrossed to a homozygous recessive male (a d/a d), what percent of the progeny are predicted to have the genotype A d/a d? A. 100 B. 75 C. 50 D. 25 E. Can’t be determined 2. Yellow body (y), vermillion eye color (v) and miniature body (m) are recessive...
here are the original strains:
please answer C, D, E, and F with full solutions, thank you!
:)
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...
2. You have performed the following mating experiment using Hfr and F"strains of Escherichia coli:(4 marks) Hfr (asp+ trp+ mal+ StrepS) x F (asp-trp-mal- Strep). Strep = streptomycin, an antibiotic Asp = aspartic acid, an amino acid Trp = tryptophan, an amino acid Mal = maltose, a sugar molecule, can be used by some organisms as an energy source rather than glucose (a) What selective media would you use to score recombinant colonies that have received all of the markers...
2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine the location of the his mutation on the E. coli chromosome, you perform interrupted mating experiments with 5 different Hfr strains. The following chart shows the time of entry (minutes, in parentheses) of the wild-type alleles of the first 5 markers (mutant genes) into the His strain. + + Hfr A. Hfr B Hfr CH Hfr D. Hfr E- his (3) cys (11)- arg...
7. Five Hfr strains A through E are derived from a single F strain of E. coli. The following chart shows the entry times in minutes of the first five markers into an F strain when each Hfr strain is used in an interrupted-conjugation experiment: A B C E pro gal mal strs ser ade his ade This gal pro mer 11 16 36 51 13 28 38 44 70 prot met xyl mal" strs 29 32 37 his 10...
This is a Hfr mapping question and I'm completely lost
here
Basically the bonus is a puzzle. It is based on mapping done with 4 different Hfr strains. I've given you the minutes that that it takes for several of the markers on the map to transfer in as determined by Hfr mapping. All you have to do is put them in the correct order around the circular chromosome and show me how many minutes are in between each marker...
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