Three Hfr strains of E. coli, each with genotype StrS, lacZ+, A, B, C, D are...
Three Hfr strains of E. coli, each with genotype StrS, lacZ+, A, B, C, D are mated to an F- strain with the genotype StrR, lacZ-, a, b, c, d. Samples of conjugating strains are collected and disrupted every minute for one hour using a blender. Cells are plated under conditions that allow the lacZ+, A, B, C, and D alleles to be identified. The time at which each dominant allele is transferred is shown in the table.
|
lacZ |
A |
B |
C |
D |
|
|
Hfr1 |
50’ |
5’ |
15’ |
40’ |
|
|
Hfr2 |
2’ |
47’ |
60’ |
37’ |
12’ |
|
Hfr3 |
35’ |
5’ |
45’ |
Which TWO genes on the final map are located immediately to the left and right of the insertion site of Hfr 1? (SELECT TWO ANSWER CHOICES.)
A) Lac Z
B) A
C) B
D) C
E) D
F) E
Homework Answers
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Three Hfr strains of E. coli, each with genotype StrS, lacZ+, A,
B, C, D are...
7. Five Hfr strains A through E are derived from a single F strain of E....
7. Five Hfr strains A through E are derived from a single F strain of E. coli. The following chart shows the entry times in minutes of the first five markers into an F strain when each Hfr strain is used in an interrupted-conjugation experiment: A B C E pro gal mal strs ser ade his ade This gal pro mer 11 16 36 51 13 28 38 44 70 prot met xyl mal" strs 29 32 37 his 10...
An Hfr strain of E. coli with genotype a+b+c+d+e+f+ is mated with an F¯ auxotrophic strain...
An Hfr strain of E. coli with genotype a+b+c+d+e+f+ is mated with an F¯ auxotrophic strain with the genotype a¯b¯c¯d¯e¯f¯. Conjugation is stopped at 10 minute intervals and the genotypes of the resulting conjugants are determined. The following results are obtained: after 10 minutes e+ after 20 minutes a+ e+ after 30 minutes a+ b+e+ after 40 minutes a+b+d+e+ after 50 minutes a+b+c+d+e+ after 60 minutes a+b+c+d+e+f+ Which represents the correct order of genes on this bacterial chromosomes? efabcd eabdcf...
1) Genetics a) Which of the following genotypes have one haplotype in common with the genotype...
1) Genetics a) Which of the following genotypes have one haplotype in common with the genotype aBcdE/ABcDe? abdeE/ABcDE ABcDE/ABCDe abCDE/aBcdE ABcde/abcde b) Consider an experiment in which an Hfr strain of E. coli bacteria with the StrS, his+, met+ alleles in its genome is mixed with an F- strain of E. coli with the StrR, his-, and met- alleles in its genome. After the cells have been cultured together for 8 hours, the mixture of cells is plated on minimal...
here are the original strains: please answer C, D, E, and F with full solutions, thank...
here are the original strains:
please answer C, D, E, and F with full solutions, thank you!
:)
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene...
Three autotrophic strains of E. coli (#1-3) of genotype a(-)b(+) are mixed in all possible combinations...
Three autotrophic strains of E. coli (#1-3) of genotype
a(-)b(+) are mixed in all possible combinations with three
auxotrophic strains (#4-6) of genotype a(+)b(-), and subsequently
plated on basal media.
A few recombinants form colonies when the following strains
are mixed: 2+4 and 3+6
Many recombinants form colonies when the following strains are
mixed: 1+6 and 2+5
All other combinations produce no surviving recombinants on
basal media.
What are the mating types (F+, F-, Hfr) of all six
strands?
Three...
You are performing an interrupted mating experiment testing for transfer of lac, gal, strR, ile, trp,...
You are performing an interrupted mating experiment testing for transfer of lac, gal, strR, ile, trp, tonR, and val. You have the following plates available to you: Plate A: MM Plate B: MM + lac + val + ile + trp Plate C: MM + gal + T1 phage Plate D: MM + val Plate E: MM + streptomycin + ile + trp Plate F: MM + lac + trp Which of the above plates selects for transfer of the...
3. (7) Suppose that you have selected E. coli that can grow only when the medium...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine...
2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine the location of the his mutation on the E. coli chromosome, you perform interrupted mating experiments with 5 different Hfr strains. The following chart shows the time of entry (minutes, in parentheses) of the wild-type alleles of the first 5 markers (mutant genes) into the His strain. + + Hfr A. Hfr B Hfr CH Hfr D. Hfr E- his (3) cys (11)- arg...
Why do different conjugating strains show different gradients of transfer? Group of answer choices A. Hfr...
Why do different conjugating strains show different gradients of transfer? Group of answer choices A. Hfr plasmids pick up different bacterial genes when they excise from the chromosome. B. There is variation in the rate that each F+ strain transfers DNA. C. The Hfr plasmid can integrate into different locations in the bacterial genome creating different F+ strains that initiate transfer at different locations. D. The F+ plasmid can integrate into different locations in the bacterial genome creating different Hfr...
33. In E. coli, a streptomycin-sensitive, prototrophic Hfr strain was mated to a streptomycin-resistant F-strain auxotrophic...
33. In E. coli, a streptomycin-sensitive, prototrophic Hfr strain was mated to a streptomycin-resistant F-strain auxotrophic for a number of nutritional requirements. After conjugation for various times, the results shown at right were obtained. Of the four genes shown, which two genes map the closest to each other? azir tonr Percent of recipients receiving a given gene lact gal + 10 50 60 20 30 40 Time (minutes)
7. Five Hfr strains A through E are derived from a single F strain of E. coli. The following chart shows the entry times in minutes of the first five markers into an F strain when each Hfr strain is used in an interrupted-conjugation experiment: A B C E pro gal mal strs ser ade his ade This gal pro mer 11 16 36 51 13 28 38 44 70 prot met xyl mal" strs 29 32 37 his 10...
here are the original strains:
please answer C, D, E, and F with full solutions, thank you!
:)
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene...
Three autotrophic strains of E. coli (#1-3) of genotype
a(-)b(+) are mixed in all possible combinations with three
auxotrophic strains (#4-6) of genotype a(+)b(-), and subsequently
plated on basal media.
A few recombinants form colonies when the following strains
are mixed: 2+4 and 3+6
Many recombinants form colonies when the following strains are
mixed: 1+6 and 2+5
All other combinations produce no surviving recombinants on
basal media.
What are the mating types (F+, F-, Hfr) of all six
strands?
Three...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine the location of the his mutation on the E. coli chromosome, you perform interrupted mating experiments with 5 different Hfr strains. The following chart shows the time of entry (minutes, in parentheses) of the wild-type alleles of the first 5 markers (mutant genes) into the His strain. + + Hfr A. Hfr B Hfr CH Hfr D. Hfr E- his (3) cys (11)- arg...
33. In E. coli, a streptomycin-sensitive, prototrophic Hfr strain was mated to a streptomycin-resistant F-strain auxotrophic for a number of nutritional requirements. After conjugation for various times, the results shown at right were obtained. Of the four genes shown, which two genes map the closest to each other? azir tonr Percent of recipients receiving a given gene lact gal + 10 50 60 20 30 40 Time (minutes)
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