Question

University switched to online classed due to CoVID-19. Needless to say I am having difficulty grasping the material as its my first "online" class. Can someone explain this to me please?

The following sequence of nucleotides in a DNA strand was used as a template to synthesize a mRNA that was then translated into protein:

                        5’-TTAACGGCTTTTTTCCAT-3’

Predict the C-term aa and the N-term aa of the resulting polypeptide.

Answer 1

Answer:

As we know that the c terminal of an amino acid is characterized by the presence of a OH group , it's best way to search for the terminal OH group to decide the C terminal.

DNA; 5'TTA ACG GCT TTT TTC CAT 3'

m RNA; 3'AAU UGC CGA AAA AAG GUA5'

AA ;C-Asn-cys-Arg-Lys-Lys- Val -N terminal

SO As we had discussed , there is always a OH at 3' position. So we can conclude that of the resulting polypeptide 3' side would be C terminal and 5' side would be N terminal. Give a thumbs up if you like the answer. Thank you