After performing ChIP-seq, you find that the protein you are working on binds to the promoter...
After performing ChIP-seq, you find that the protein you are working on binds to the promoter and just into the gene body of many genes throughout the genome. You hypothesize this protein may be important for RNA Pol II pausing and you want to gain nucleotide resolution of the transcripts this protein directly regulates. After depleting this protein, what technique would you perform to determine, at nucleotide resolution, the transcription and potential change to RNA Pol II pausing resulting loss of this protein?
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After performing ChIP-seq, you find that the protein you are
working on binds to the promoter...
Which technique would you use if you wanted to < Identify all the regions of open...
Which technique would you use if you wanted to < Identify all the regions of open chromatin in a particular cell type? Perform a paternity test? Amplify a specific sequence of DNA? [Choose] [Choose] Promoter fusion DNAse-seq Scanning mutagenesis Western blot RNAseq DNA fingerprinting Northern blot Reporter Assay DNAse footprinting ChIP-PCR EMSA Southern blot DNAse protection assay PCR BLAST search Protein fusion ChIP-seq [Choose Compare the global level of RNA expression between two samples? Detect presence of a specific protein...
You and your lab mate, Eugene Yous, are performing expression-profiling experiments using RNA-Seq. You have extracted...
You and your lab mate, Eugene Yous, are performing
expression-profiling experiments using RNA-Seq. You have extracted
mRNA from a mouse liver. Both you and Eugene profile the same exact
mRNA sample, but you decide to use polyT primer to make your cDNA
whereas Eugene decides to use random priming. You obtain the exact
same results across the genome except at one locus, the gene Ipt25.
You find 250 reads map to Ipt25 whereas Eugene finds 45,000 reads
mapping to Ipt25....
can someone explain how to answer this with reasons why 30-45 mins after estrogen addition: Acchyiase...
can someone explain how to answer this with reasons why
30-45 mins after estrogen addition: Acchyiase (HAT, odde auhye 60-90 mins after estrogen addition: Reauitment ef Poy to open 7. T identify the Gene X DNA element responsible for regulation by a Growth Fagor, you perform a linker scanning experiment in which you mutate 10 bp Tegions centered around positions -205 to-5 in the Gene X promoter/promoter- proximal region (wt wild-type promoter). The promoter variants were tested for their ability...
can you guys please give me the correct answers and explain why? 19. You clone your...
can you guys please give me the correct answers and explain
why?
19. You clone your favorite E. coli gene including the promoter region, the open reading frame, and some flanking DNA. You determine the DNA sequence of the entire region and map the start site of transcription. You note the following sequence near the end o your gene. What is the likely function of this sequence? ...CCCAGCCCGCCTAATGAGCGGGCTTTTTTTTTTGAAGGTATAT... A. A polyadenylation signal B. A Rut site C. The ribosome binding...
4. The CRISPR-Cas9 system is an important new technique in molecular biology. What is the natural...
4. The CRISPR-Cas9 system is an important new technique in
molecular biology. What is the natural function of this system?
Describe how you would use this system to generate a null mutation
in another organism (i.e. explain Figure 6-43). How does it work?
What is the modification of the method that allows for correction
of a mutation (e.g. the mouse crystalline gene)? And lastly, what
are the problems with the CRISPR system?
FIGURE 6-43 Single-nucleotide mutations
can be introduced into...
PLEASE ANSWER ALL THE QUESTIONS: 1.What is true of tRNA (transfer RNA)? A they contain an...
PLEASE ANSWER ALL THE QUESTIONS: 1.What is true of tRNA (transfer RNA)? A they contain an anti-codon B they carry an amino acid C they can interpret the genetic code D all of these are true 2. How can transcription factors bound to distant enhancers influence gene expression? A the transcription factors can slide along the DNA until they get to the gene's promoter B DNA can loop, bringing these proteins into contact with the gene's promoter C both of...
Please ignore short answer and fill in the blank question. Matching (15 points) 15questions (only write...
Please ignore short answer and fill in the blank question.
Matching (15 points) 15questions (only write ONE letter that fits BEST) _1. FtsZ 2.MreB 3. Divisome 4. Chemostat 5.Batch culture 6. Alkaliphile 7. Acidophile 8. Obligate anaerobe 9. Facultative anaerobe 10. Microaerophile 11. Micronutrients 12. Macronutrients _13. Selective media _14. Defined media 15. Complex media Short answers (X points) A. determines shape of cell B. a protein which initiates cell division C. continuous culture D. complex of proteins that directs...
Need help filling in the chart and answering the questions that go along with it. I...
Need help filling in the chart and answering the questions
that go along with it. I have added the procedure and the
instructions as well as the "results" that are supposed to be used
to fill in the chart. Thank you!
We were unable to transcribe this imageTABLE 8-1 Cast of Characters and a Legend of Abbreviations Name Symbol Function in This Experiment Green fluorescent protein GFP It serves as an indicator of successful transformation and gene transcription expression in...
Which technique would you use if you wanted to < Identify all the regions of open chromatin in a particular cell type? Perform a paternity test? Amplify a specific sequence of DNA? [Choose] [Choose] Promoter fusion DNAse-seq Scanning mutagenesis Western blot RNAseq DNA fingerprinting Northern blot Reporter Assay DNAse footprinting ChIP-PCR EMSA Southern blot DNAse protection assay PCR BLAST search Protein fusion ChIP-seq [Choose Compare the global level of RNA expression between two samples? Detect presence of a specific protein...
You and your lab mate, Eugene Yous, are performing
expression-profiling experiments using RNA-Seq. You have extracted
mRNA from a mouse liver. Both you and Eugene profile the same exact
mRNA sample, but you decide to use polyT primer to make your cDNA
whereas Eugene decides to use random priming. You obtain the exact
same results across the genome except at one locus, the gene Ipt25.
You find 250 reads map to Ipt25 whereas Eugene finds 45,000 reads
mapping to Ipt25....
can someone explain how to answer this with reasons why
30-45 mins after estrogen addition: Acchyiase (HAT, odde auhye 60-90 mins after estrogen addition: Reauitment ef Poy to open 7. T identify the Gene X DNA element responsible for regulation by a Growth Fagor, you perform a linker scanning experiment in which you mutate 10 bp Tegions centered around positions -205 to-5 in the Gene X promoter/promoter- proximal region (wt wild-type promoter). The promoter variants were tested for their ability...
can you guys please give me the correct answers and explain
why?
19. You clone your favorite E. coli gene including the promoter region, the open reading frame, and some flanking DNA. You determine the DNA sequence of the entire region and map the start site of transcription. You note the following sequence near the end o your gene. What is the likely function of this sequence? ...CCCAGCCCGCCTAATGAGCGGGCTTTTTTTTTTGAAGGTATAT... A. A polyadenylation signal B. A Rut site C. The ribosome binding...
4. The CRISPR-Cas9 system is an important new technique in
molecular biology. What is the natural function of this system?
Describe how you would use this system to generate a null mutation
in another organism (i.e. explain Figure 6-43). How does it work?
What is the modification of the method that allows for correction
of a mutation (e.g. the mouse crystalline gene)? And lastly, what
are the problems with the CRISPR system?
FIGURE 6-43 Single-nucleotide mutations
can be introduced into...
Please ignore short answer and fill in the blank question.
Matching (15 points) 15questions (only write ONE letter that fits BEST) _1. FtsZ 2.MreB 3. Divisome 4. Chemostat 5.Batch culture 6. Alkaliphile 7. Acidophile 8. Obligate anaerobe 9. Facultative anaerobe 10. Microaerophile 11. Micronutrients 12. Macronutrients _13. Selective media _14. Defined media 15. Complex media Short answers (X points) A. determines shape of cell B. a protein which initiates cell division C. continuous culture D. complex of proteins that directs...
Need help filling in the chart and answering the questions
that go along with it. I have added the procedure and the
instructions as well as the "results" that are supposed to be used
to fill in the chart. Thank you!
We were unable to transcribe this imageTABLE 8-1 Cast of Characters and a Legend of Abbreviations Name Symbol Function in This Experiment Green fluorescent protein GFP It serves as an indicator of successful transformation and gene transcription expression in...
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