Question

6. Analyze the graph. sS DNA dsDNA 0 30 50 70 90 110 Temperature c) A. Define Tm. rovide the value for Im in the grap C. At what point in the graph is full separation of dsDNA achieved?

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Answer:

A.Temperature which results in melting of double stranded DNA into 50% of the single stranded DNA is called melting temperature of DNA.

B. Tm from the graph is 70C

C. At 90C+ all of the DNA in the mix will be ss DNA.

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