Question

12. During the annealing cycle, if the annealing temperature is too low: a. Very low quantity of amplicon is yielded b. Non specific amplification can occur c. The number of cycles must be redone d. The DNA sample is degraded 13. In PCR cycles, by the end of each cycle, the copy number of amplicon is nearly a Tripled b. Doubled c. Decreased d. Quadrupled 14. PCR amplification is carried out by which of the following instruments: a. Thermal cycler b. Thermal tumbler c. Multiplex d. All of the above Chapter 7 Additional Study Questions 15. When two alleles in a heterozygous individual are unequally detected at a low level of starting DNA template, it is referred to as: a. The Stochastic Effect b. PCR degridation C. PCR inhibition d. Both a ande 16. Which of the following is an example of a PCR inhibitor a. Indigo dyes b. mtDNA CRNA d. STR 17. Please choose which of the following is an example of a control to monitor PCR contamination: a. Reagent blanks b. Negative controls c. RNA d. Both a and b 18. This is defined as a linear molecule containing four types of nucleotides linked together by phosphdiester bonds: a Polymerase Chain Reaction b. Short Tandem Repeat C. Ribonucleic acid d. Reverse Transcriptase-PCR 19. Who coined the term "central dogma." a Francis Crick b. James Watson c. Kary Mullis d. None of the above copies 20. Reverse Transcriptase-PCR is a method used to amplify of RNA a. RT-PCR b. cDNA cmNa d PCR

Answer 1

12. When the annealing temperature is too low, the primer will bind non specifically. This will result in non-specific amplification.

Option 'b' non-specific amplification.

13. Copy number amplification is

b..Doubled.

The number of DNA gets doubled in each cycle giving huge numbers of DNA copies.

14. PCR amplification is carried out by

a. Thermal cycler.

Thermal cycler increases and decreases the temperature in a cyclic manner to denature and anneal the DNA.

15. Heterozygous alleles are unequally detected

A. Stochastic effect.

Failure to detect one or both alleles will result in unequal amplification. So we see unequal peaks during amplification.