Question

.Select the probe sequence that will hybridize to the following nucleic acid sequence:
C G A T A T T G T C

A. T A G T A C A A G A

B. C G A T A T T G T C

C. G T C A A G A C C T

D G C T A T A A C A G

Select the strand of RNA that is complementary to this single strand of DNA:
A T T A G C C G T A T

A. T A A T C G G C A T A

B. U A A U C G G C U A U

C. T U U T C G G C A U A

D. T A A T U G G U A T A

60. What process confers specificity in molecular biology methods?

A. Amplification

B. Polymorphism

C. Strand cleavage

D. Hybridization

In a polymerase chain reaction, the strand length of the amplicons is determined by the:

A. number of cycles.

B. percentage of cycle time spent in the extension step.

C. primer sequences.

D. total cycle time.

The common characteristic of transcription-mediated amplification and reverse-transcriptase polymerase chain reaction is that both:

A. are isothermal processes.

B. start with an RNA target.

C. use DNA polymerase.

D. generate RNA as the primary product.

Sanger chain termination sequencing differs from polymerase chain reaction because:

A. only three nucleotide bases are used.

B. DNA polymerase is not needed.

C. only one primer is used.

D. the process is isothermal.

When denatured, DNA is converted from:

A. a double-stranded double helix to a double-stranded linear structure.

B. a double strand to a pair of single strands.

C. DNA to RNA.

D. polymerized strands of nucleic acids to individual nucleic acids.

In the process of translation, the addition of a particular amino acid to a growing protein may be specified by:

A. one specific mRNA nucleotide.

B. only one specific combination of three mRNA nucleotides.

C. one of several specific combinations of three mRNA nucleotides.

D. polymerized strands of nucleic acids to individual nucleic acids.

Answer 1

1.amplification

2.number of cycles

3. Use DNA polymerase

4.only one primer is used

5.polymerized strands of nucleic acids to individual nucleic acids.