Question

9. An alteration in the nucleic acid sequence in which a specific restriction endonuclease cuts can be detected by a. ELISA b. flow cytometry c. RFLP d. FISH

10. Which of the following techniques enables the identification of a particular sequence of nucleic acid on a cell or tissue? a. northern blot b. western Blot c. FISH d. southern blot

11. The polymerase chain reaction is used to a. convert RNA back into DNA b. amplify a target nucleic acid sequence to obtain large amounts of that sequence c. isolate DNA from a DNA/RNA mixture d. identify specific amino acid sequences of a section of a protein

12. Denaturation or "melting" DNA refers to a. converting DNA into a liquid form so it can be separated by electrophoresis b. creating RNA/DNA hybrid molecules c. separating a DNA sequence into individual amino acid codons d. separating double-stranded DNA into single strands

13. The process of allowing single-stranded DNA to re-form into a double-stranded form is called a. annealing b. degradation c. denaturation d. amplification

Answer 1

Ansa._- RELP Exeplainatton - Restriction fragment length polymorphism (RFLP) is a process in which variation of DNA us_cut by restriction enzyme._ELISA, How cytometry and ASH is a technique which is antibodiy based Anslo - Option c - FISH FISH technique is specifically used in the cell and_Hosue. Other technique is doesnot give information about the location of the DNA sequence in particular cell tissule, Hence other ans are wrong. Ans 11 - Option b - amplify the target nucleic acid sequence to obtain large amount of that sequence PCR is common technique for DNA amplification. RNA back into DNA is B reverse transceiption process PCR is not eased in option c and d. Answer 12 - d. seperating double stranded DNA. into single strande In PCR, Denateration is a process in which ds DNA is_p breaked into SS DNA by applying heat RNA -DNA hybrid is not denaturation Answer 13 - d. Amplification In PCR, the amplification of DNA is done by denaturation of ds DNA into ss DNA and later annealing it by adding primer. In | degradation ds DNA is degraded in SSDNA. CS$canned with CamScanner