Homework Answers
(a) Double stranded DNA
Explanation-Restriction enzymes recognize and bind to specific sequences or palindromic sequences of DNA called restriction sites.
e.g. EcoRI cuts at G/AATTC
(b) Double stranded RNA
Explanation- Dicer cleaves double stranded RNA. It is a member of ribonuclease III family (RNaase). Dicer cuts double stranded RNA into small interfering RNA (siRNA) which degrades the mRNA. This process of gene silencing is termed as RNA interference.
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(2 points). Which of the following is cut by restriction...
Please choose from this list to fill in the blanks below. Some choices can be used more...
Please choose from this list to fill in the blanks below. Some choices can be used more than once or not at all. Choices: rRNA, tRNA, miRNA, mRNA Typically forms internal base pairs to form a “cloverleaf” structure _________________ The primary transcript is larger than the final, mature form _____________________ Associates with proteins to form the ribosome ________________________ Drosha is needed to process this form of RNA ______________________ Contains protein-coding regions _________________________ This form of RNA contains the largest variety of nucleotide modifications ________________...
Which is not true about restriction enzymes? a. They cut RNA b. They evolved as a...
Which is not true about restriction enzymes? a. They cut RNA b. They evolved as a bacteria defense mechanism c. They can leave single-stranded overhanging sequences called Sticky ends d. They recognize a specific target sequence e.They cut DNA
Are the following statements true or false? Please explain your answers. 1. Restriction nucleases cut DNA...
Are the following statements true or false? Please explain your answers. 1. Restriction nucleases cut DNA at specific sites that are always located between genes. 2. DNA migrates toward the positive electrode during electrophoresis. 3. Clones isolated from cDNA libraries contain promoter sequences. 4. PCR utilizes a heat-stable DNA polymerase because for each amplification step, double-stranded DNA must be heat-denatured.
DNA fragments cut by most restriction enzymes have: double-stranded complementary ends. either only sequences of Gs...
DNA fragments cut by most restriction enzymes have: double-stranded complementary ends. either only sequences of Gs or only sequences of Cs. cuts made at random points along one of the strands. protruding sticky ends.
9. An alteration in the nucleic acid sequence in which a specific restriction endonuclease cuts can...
9. An alteration in the nucleic acid sequence in which a specific restriction endonuclease cuts can be detected by a. ELISA b. flow cytometry c. RFLP d. FISH 10. Which of the following techniques enables the identification of a particular sequence of nucleic acid on a cell or tissue? a. northern blot b. western Blot c. FISH d. southern blot 11. The polymerase chain reaction is used to a. convert RNA back into DNA b. amplify a target nucleic acid...
If you cut the following single stranded DNA fragment with a restriction enzyme with restriction site...
If you cut the following single stranded DNA fragment with a restriction enzyme with restriction site of 5’GAATTC 3” and the cutting point between G and A. a. How many fragments you will get b. Specify the size (Number of bases) and the sequence of each fragment, pay attention to DNA direction (5’-3’) 5” ACATTGTCCGGGAATTC CGGGCTAGGCAT T GAATTGGAACA GAATTC GGGCCCGATCCGTA 3
1. A circular plasmid has two PmeI restriction sites. A PmeI restriction enzyme will cut this...
1. A circular plasmid has two PmeI restriction sites. A PmeI restriction enzyme will cut this plasmid into two fragments. A. True B. False 2.In general, restriction enzymes that recognize four nucleotides have higher probability to produce more DNA fragments than those enzymes that recognize six nucleotides. A. true B. false 3. Which of the following sequences are palindromes? A. 5' TGGCCA 3' B. 5' GAAAAG 3' C. 5' CGATGG 3' D. 5' GACGAC 3' 4. Below are the possible...
14. Restriction endonucleases are a. enzymes that restrict DNA synthesis b. enzymes that cut DNA in...
14. Restriction endonucleases are a. enzymes that restrict DNA synthesis b. enzymes that cut DNA in specific sequences c. nuclear proteins that are involved in transcription d. components of the ribosomes involved in protein synthesis 15. The first step in southern blotting is a. converting DNA into RNA b. cutting high molecular weight DNA into smaller pieces c. converting RNA into DNA d. radioactively labeling the DNA so it can be detected after the procedure is complete 16. The major...
emaining: 1:34:44 Show Time Remaining 3 of 5 - Multiple Choice Questions: Please select the best...
emaining: 1:34:44 Show Time Remaining 3 of 5 - Multiple Choice Questions: Please select the best answer that addresses each question (2 pol Question 24 of 33 2 Points Which of the following is not true about the viral reverse transcriptase? A. It can use a single stranded DNA as a template to synthesize a double stranded DNA. B. It has ribonuclease activity. C. It has an RNA-dependent DNA polymerase activity D. It has a promiscuous specificity for nucleotides. E....
Write neat please and box the final answer please D Question 3 0.5 pts The RNA...
Write neat please and box the final answer please
D Question 3 0.5 pts The RNA primers used to initiate replication in E.coli are removed by helicase + ATP. are joined together by DNA ligase result in Okazaki fragments on the leading strand. are removed by DNA Polymerase! Question 4 0.5 pts A nucleic acid was analyzed and found to contain 30 percent A. 20 percent G, 15 percent C, and 35 percent T. The nucleic acid must be double-stranded...
emaining: 1:34:44 Show Time Remaining 3 of 5 - Multiple Choice Questions: Please select the best answer that addresses each question (2 pol Question 24 of 33 2 Points Which of the following is not true about the viral reverse transcriptase? A. It can use a single stranded DNA as a template to synthesize a double stranded DNA. B. It has ribonuclease activity. C. It has an RNA-dependent DNA polymerase activity D. It has a promiscuous specificity for nucleotides. E....
Write neat please and box the final answer please
D Question 3 0.5 pts The RNA primers used to initiate replication in E.coli are removed by helicase + ATP. are joined together by DNA ligase result in Okazaki fragments on the leading strand. are removed by DNA Polymerase! Question 4 0.5 pts A nucleic acid was analyzed and found to contain 30 percent A. 20 percent G, 15 percent C, and 35 percent T. The nucleic acid must be double-stranded...
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