Are the following statements true or false? Please explain your answers. 1. Restriction nucleases cut DNA...
Are the following statements true or false? Please explain your answers.
1. Restriction nucleases cut DNA at specific sites that are always located between genes.
2. DNA migrates toward the positive electrode during electrophoresis.
3. Clones isolated from cDNA libraries contain promoter sequences.
4. PCR utilizes a heat-stable DNA polymerase because for each amplification step, double-stranded DNA must be heat-denatured.
Homework Answers
1. True, restriction endonuclease cut DNA at specific sites.
2. True, the negative charge of the sugar phosphate backbone enables the DNA to migrate towards the positive electrode during the process of electrophoresis.
3. False, cDNA does not possess any promotor sequence because it is made with the help of mRNA template.
4. True, DNA polymerase used in PCR reactions should tolerate high temperature. Taq DNA polymerase is most commonly used.
Add Answer to:
Are the following statements true or false? Please explain your
answers.
1. Restriction nucleases cut DNA...
Write true or false ______ 1. The DNA sequence of one human being is on average...
Write true or false ______ 1. The DNA sequence of one human being is on average 99.9% identical to another random human being. ______ 2. As of 2009, all living human beings have had their entire genome sequenced. ______ 3. The nucleotide bases present in a DNA sequence are A, U, G, C. ______ 4. Techniques that enabled scientists to clone genes were developed in the 1970s. ______ 5. A restriction enzyme is useful because it is a generic enzyme...
NEED HELP WITH THESE QUESTIONS. PLEASE ANSWER ALL AND EXPLAIN AS WELL. THANKSSSSSSS 1. You want...
NEED HELP WITH THESE QUESTIONS. PLEASE ANSWER ALL AND EXPLAIN AS
WELL. THANKSSSSSSS
1. You want to clone a gene from a donor vector to a host vector. List the correct order of events in the process of cloning a. Perform ligation reaction of cloned gene and host vector. b. Perform double digestion of both donor and host vectors with the 2 restriction enzymes c. Examine donor and host vectors for restriction sites d. Purify cloned gene from donor vector...
14. Which of the following statements is true of lipids? A) Many contain fatty acids in...
14. Which of the following statements is true of lipids? A) Many contain fatty acids in ester or amide linkage. B) Most are simply polymers of isoprene C) Testosterone is an important sphingolipid found in myelin. D) They are more soluble in water than in chloroform. E) They play only passive roles as energy-storage molecules. 15. The sigma factor of E. coli RNA polymerase: A) associates with the promoter before binding core enzyme. B) combines with the core enzyme to...
please answer all amils A Doon E Conjagants DNA hips o sloning of freign DA fa...
please answer all
amils A Doon E Conjagants DNA hips o sloning of freign DA fa ts are called C Clne D Vecto 20 Whatkind of termini is produced by the retriction endonelease Prl, which restriction site has is 5CGATIC (1he dowswd aow represents the site of cleavage in each strand) D Reverse ends E Polylinker termini A.Y ovehan Byaverhang C Blant ends 21.Eeryme dhat cleave DNA at seuence-specifie sites is called D Exonuclease E Integrase A DNA polymerase Ligase...
1. An enzyme used to covalently join DNA segments to form recombinant DNA molecules is called...
1. An enzyme used to covalently join DNA segments to form recombinant DNA molecules is called a A. Restriction endonuclease B. Reverse transcriptase C. DNA polymerase D. Helicase E. Ligase 7. The procedure for introducing changes into specific genes is called A. An enhancer trap B. Imprinting C. RT-PCR D. DNA looping E. Gene targeting 2. Plasmids used for in vitro cloning of foreign DNA fragments are called A. Donors B. DNA chips C. Clones D. Vectors E. Conjugants 8....
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteina...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
and w Two-dimensional gel electrophoresis separates proteins based on a. shape; charge Ob.size; concentration c. concentration;...
and w Two-dimensional gel electrophoresis separates proteins based on a. shape; charge Ob.size; concentration c. concentration; shape O d. size, charge O e. size; shape Refer to the table. Several strains of a bacterium are sequenced to investigate the pan and core genomes. In the table, + denotes presence of the gene and denotes its absence. Gene Gene Gene Gene Gene Strain ! Strain 2 + Strain 3 + Strain 4 + + + + Strain 5 + + What...
NEED HELP WITH THESE QUESTIONS. PLEASE ANSWER ALL AND EXPLAIN AS
WELL. THANKSSSSSSS
1. You want to clone a gene from a donor vector to a host vector. List the correct order of events in the process of cloning a. Perform ligation reaction of cloned gene and host vector. b. Perform double digestion of both donor and host vectors with the 2 restriction enzymes c. Examine donor and host vectors for restriction sites d. Purify cloned gene from donor vector...
14. Which of the following statements is true of lipids? A) Many contain fatty acids in ester or amide linkage. B) Most are simply polymers of isoprene C) Testosterone is an important sphingolipid found in myelin. D) They are more soluble in water than in chloroform. E) They play only passive roles as energy-storage molecules. 15. The sigma factor of E. coli RNA polymerase: A) associates with the promoter before binding core enzyme. B) combines with the core enzyme to...
please answer all
amils A Doon E Conjagants DNA hips o sloning of freign DA fa ts are called C Clne D Vecto 20 Whatkind of termini is produced by the retriction endonelease Prl, which restriction site has is 5CGATIC (1he dowswd aow represents the site of cleavage in each strand) D Reverse ends E Polylinker termini A.Y ovehan Byaverhang C Blant ends 21.Eeryme dhat cleave DNA at seuence-specifie sites is called D Exonuclease E Integrase A DNA polymerase Ligase...
1. An enzyme used to covalently join DNA segments to form recombinant DNA molecules is called a A. Restriction endonuclease B. Reverse transcriptase C. DNA polymerase D. Helicase E. Ligase 7. The procedure for introducing changes into specific genes is called A. An enhancer trap B. Imprinting C. RT-PCR D. DNA looping E. Gene targeting 2. Plasmids used for in vitro cloning of foreign DNA fragments are called A. Donors B. DNA chips C. Clones D. Vectors E. Conjugants 8....
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
and w Two-dimensional gel electrophoresis separates proteins based on a. shape; charge Ob.size; concentration c. concentration; shape O d. size, charge O e. size; shape Refer to the table. Several strains of a bacterium are sequenced to investigate the pan and core genomes. In the table, + denotes presence of the gene and denotes its absence. Gene Gene Gene Gene Gene Strain ! Strain 2 + Strain 3 + Strain 4 + + + + Strain 5 + + What...
Most questions answered within 3 hours.
-
John and his friends wanted to see how their friends compared to
the reading habits of...
asked 1 minute from now -
When we have two sorted lists of numbers in non-descending
order, and we need to merge...
asked 22 seconds ago -
Find the variance for the given data. Round your answer to one
more decimal place than...
asked 13 minutes ago -
Decision #2: Planning for
Retirement
Luke and Olivia are 22, newly married, and ready to embark...
asked 7 minutes ago -
Which one of these statements is correct concerning the time
value of money?
Decreasing the PV...
asked 7 minutes ago -
Imagine a PPF for two goods: guns and butter. A natural disaster
hits and reduces our...
asked 8 minutes ago -
Two wavefronts that add with constructive amplitude
reinforcement, have what property?
There are 270° out of...
asked 11 minutes ago -
Electromagnetic radiation of wavelength 96.4 nm was projected on
a zinc plate with a 6.01×10-19 J...
asked 29 minutes ago -
5. Explain what happens when the dose is larger than the
threshold dose.
6. Explain how...
asked 21 minutes ago -
I have a few concentrations that are in mg/L and I need to
calculate them in...
asked 23 minutes ago -
My son, daughter and dog have successfully built a storm-making
machine! They've created a storm that...
asked 29 minutes ago -
Q. The neighborhood kids set up an outdoor lemonade stand in
Maryland in June. They find...
asked 42 minutes ago