Question

Please choose from this list to fill in the blanks below.  Some choices can be used more than once or not at all.  

            Choices:     rRNA, tRNA,  miRNA,  mRNA

Typically forms internal base pairs to form a “cloverleaf” structure _________________

The primary transcript is larger than the final, mature form _____________________

Associates with proteins to form the ribosome ________________________

Drosha is needed to process this form of RNA ______________________

Contains protein-coding regions _________________________

This form of RNA contains the largest variety of nucleotide modifications ________________

snoRNAs are used to guide modifications of this form of RNA __________________

May be found within introns of protein-coding RNAs _______________________

Processing involves the snRNAs (U RNAs) ____________________________

Formation of this RNA may involve removal of large and numerous introns __________

A2. (2 points).    Which of the following is cut by restriction enzymes?

1. Double-stranded DNA       b. Double stranded RNA     c. Single-stranded DNA

d. Single-stranded RNA

A3.  (2 points). Which of the following is cut by Dicer?

1. Double-stranded DNA     b. Double stranded RNA     c. Single-stranded DNA

d. Single-stranded RNA

  

A4. (6 points).  Please state which molecule is found at each of the three locations indicated above.  

               2-O-methyl ribose,   7 methyl guanosine,  N6 methyl adenosine

1. _______________________
2. ______________________
3. _______________________

Answer 1

A1. a) Typically forms internal base pairs to form a “cloverleaf” structure tRNA.

b) The primary transcript is larger than the final, mature form mRNA.

c) Associates with proteins to form the ribosome rRNA.

d) Drosha is needed to process this form of RNA miRNA.

e) Contains protein-coding regions mRNA.

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