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Which of the following make(s) the Southern blot technique possible? (Select all that apply.) A) New...

Which of the following make(s) the Southern blot technique possible? (Select all that apply.) A) New nucleotides are added only to the 3' end of a growing DNA strand. B) A DNA strand whose 3' end terminates in a dideoxynucleotide cannot be elongated. C) Complementary single-stranded nucleic acid sequences can come together to form a duplex molecule. D) Duplex nucleic acid molecules can be separated by size by means of electrophoresis. E) Single-stranded nucleic acid molecules can be immobilized on certain types of filter paper.

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Answer #1

Answer: Options C, D, and E are correct
Explanation:
Southern blotting: Detection of DNA using complementary probe sequence
A) New nucleotides are added only to the 3' end of a growing DNA strand: Southern blotting does not utilize strand extension
B) A DNA strand whose 3' end terminates in a dideoxynucleotide cannot be elongated: Southern blotting does not utilize ddNTPs and chain termination
C) Complementary single-stranded nucleic acid sequences can come together to form a duplex molecule: A probe is used to detect the presence of a complementary sequence
D) Duplex nucleic acid molecules can be separated by size by means of electrophoresis: A gel is run to separate the DNA fragments based on their size
E) Single-stranded nucleic acid molecules can be immobilized on certain types of filter paper: Nucleic acids are immobilized onto the nitrocellulose paper

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