Question

The following diagram represents a replication bubble associated with DNA synthesis. Based on this diagram, select all of the options below that are true.
1 | 2 ---- Quadrants 1 and 4 are associated with lagging strand synthesis Quadrants 1 and 4 are associated with leading strand synthesis Quadrants 1 and 2 are associated with lagging strand synthesis Quadrants 3 and 4 are associated with lagging strand synthesis Synthesis of both daughter strands is completely continuous Telomerase activity is needed in quadrants 2 and 3 in order to extend the 3' ends of the new polynucleotide chains Telomerase activity is needed in quadrants 1 and 4 in order to extend the 3' ends of the new polynucleotide chains Telomerase activity is needed in quadrants 1 and 4 in order to extend the 5' ends of the new polynucleotide chains Quadrants 2 and 3 contain Okazaki fragments Quadrants 1 and 4 contain Okazaki fragments A single DNA primer is needed for new strand synthesis in quadrants 1 and 4 Multiple RNA primers are needed for new strand synthesis in quadrants 1 and 4

Answer 1

1. DNA replication exhibits three properties
i. Semi-conservative
ii. Semi-discontinuous
iii. Bidirectional

DNA replication occurs in 5'-3' direction.

True statements:
Quadrant 2 and 3 = Leading strand synthesis
Quadrant 1 and 4 = Lagging strand synthesis
Quadrant 1 and 4 = Telomerase activity is required in order to extend the 5'-ends of the new polynucleotide chains
Quadrant 1 and 4 = Contain Okazaki fragments

All primers made during DNA replication are RNA primers.

A single RNA primer is required in Quadrants 2 and 3.
Multiple RNA primers are required in Quadrants 1 and 4.