Which of the following is true regarding DNA sequencing? (multiple answers possible) a. Next generation sequencing...
Which of the following is true regarding DNA sequencing? (multiple answers possible)
a. Next generation sequencing is limited to simultaneous sequencing of 23 different nucleotide chains at a time
b. Next generation sequencing or “sequencing by synthesis”, requires ddNTPs
c. DNA fragments generated by the Sanger sequencing reaction each contain a primer incorporated at the 5’ end of the nucleotide chain.
d. Primers are labeled with different flurophores allowing all 4 chain termination reactions for Sanger sequencing to be combined into one tube prior to separation by capillary electrophoresis.
Homework Answers
Answer:- The correct answer is option C,DNA fragments generated by the Sanger sequencing reaction each contain a primer incorporated at the 5' end of the nucleotide chain.
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Which of the following is true regarding DNA sequencing?
(multiple answers possible)
a. Next generation sequencing...
The following diagram represents a replication bubble associated with DNA synthesis. Based on this diagram, select...
The following diagram represents a replication bubble associated
with DNA synthesis. Based on this diagram, select all of the
options below that are true.
1 | 2 ---- Quadrants 1 and 4 are associated with lagging strand synthesis Quadrants 1 and 4 are associated with leading strand synthesis Quadrants 1 and 2 are associated with lagging strand synthesis Quadrants 3 and 4 are associated with lagging strand synthesis Synthesis of both daughter strands is completely continuous Telomerase activity is needed...
Why are next-generation DNA sequencing technologies known as sequencing-by-synthesis? Why are next-generation DNA sequencing technologies known...
Why are next-generation DNA sequencing technologies known as sequencing-by-synthesis? Why are next-generation DNA sequencing technologies known as sequencing-by-synthesis? The complete DNA strands are synthesized then sequenced. Numerous synthesized fragments of DNA are sequenced to determine which nucleotides were incorporated. The sequencing occurs during S phase of the cell cycle. RNA molecules are synthesized off of the DNA templates and the incorporated nucleotides are then determined. Incorporated nucleotides are determined while they are being added to a growing DNA strand.
The Sanger method of DNA sequencing uses which chain-terminating ingredient to separate fragments differing by a...
The Sanger method of DNA sequencing uses which chain-terminating ingredient to separate fragments differing by a single nucleotide a. Magnesium b. Heat c. ddNTPs d. restriction enzymes e. none of the above
Sanger sequencing allows the identification of the bases and their order in a target piece of...
Sanger sequencing allows the identification of the bases and their order in a target piece of DNA. This technique exploits the way DNA is transcribed by using a primer and allowing new DNA to be created from its 3' end. In this technique, DNA is manufactured in vitro, which means that enzymes and molecules are combined in a test tube to allow the reactions to occur. In the original technique, four tubes were prepared, each containing the same reactants, but...
please help with all three! For DNA synthesis to take place in a Sanger sequencing reaction,...
please help with all three!
For DNA synthesis to take place in a Sanger sequencing reaction, the DNA polymerase must catalyze a reaction between the 3'- the last nucleotide and the 5- of the next nucleotide to be added. hydroxyl group, phosphate group O phosphate group, hydroxyl group O hydroxyl group, hydroxyl group O phosphate group, phosphate group QUESTION 6 millions of individual DNA fragments are isolated and sequenced in parallel during each machine run. O traditional whole-genome sequencing O...
How has DNA-sequencing technology evolved in response to the emerging needs of genome scientists? Drag the...
How has DNA-sequencing technology evolved in response to the emerging needs of genome scientists? Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Not all terms will be used. Reset Help reduce speed sequencing methods, a single serves as a template. In one such strategy, the DNA polymerase is tethered in a nanopore and fluorescently labeled nucleotides are detected as they are incorporated. The overall goal is to and accuracy of sequencing...
1. Compare and contrast first generation sequencing techniques (Sanger sequencing and pyrosequencing) and the second generation...
1. Compare and contrast first generation sequencing techniques (Sanger sequencing and pyrosequencing) and the second generation sequencing technique ForenSeq using the MiSeq. Include a discussion of reagents, time, cost, optimal sequence length, detection technologies, data output and post-testing analysis in your discussion. Tabulate your answers and be sure to complete the table. 2. Describe the processes that are occurring in PCR1 and PCR2. Why are each of these steps important. Describe the roles of i5 and i7, forward and reverse...
Next-generation sequencing involves: A. generating many short sequences from an intact, continuous DNA sequence. B. generating...
Next-generation sequencing involves: A. generating many short sequences from an intact, continuous DNA sequence. B. generating many short sequences from fragmented DNA. C. splicing together DNA fragments. D. adding multiple probes to fragmented DNA. What is the difference between a mutation and a polymorphism? A. A mutation can exist in a single person; a polymorphism must exist in at least 2% of the population. B. A mutation involves a single base pair; a polymorphism involves many base pairs. C. Mutations...
In DNA sequencing, ddNTPs differ from dNTPs in that ddNTPs are lacking a OH at the...
In DNA sequencing, ddNTPs differ from dNTPs in that ddNTPs are
lacking a OH at the
a.
2' carbon
b.
5' carbon
c.
3' carbon
please answer as many as you can!! I am low on questions and
could use the help!
Mother || Child II Male 1 Male 2 Results from a paternity test using DNA fingerprinting is shown. DNA was isolated from a mother, her child and 2 potential fathers. Primers designed to amplify different satellite DNA regions...
Which of the following make(s) sequencing by the Sanger chain-termination method possible? (Selec...
Which of the following make(s) sequencing by the Sanger chain-termination method possible? (Select all that apply.)12.17 a. Complementary single-stranded nucleic acid sequences can come together to form a duplex molecule. b. Single-stranded nucleic acid molecules can be immobilized on certain types of filter paper. c. A DNA strand whose 3' end terminates in a dideoxynucleotide cannot be elongated. d. Duplex nucleic acid molecules can be separated by size by means of electrophoresis. e. New nucleotides are added only to the...
The following diagram represents a replication bubble associated
with DNA synthesis. Based on this diagram, select all of the
options below that are true.
1 | 2 ---- Quadrants 1 and 4 are associated with lagging strand synthesis Quadrants 1 and 4 are associated with leading strand synthesis Quadrants 1 and 2 are associated with lagging strand synthesis Quadrants 3 and 4 are associated with lagging strand synthesis Synthesis of both daughter strands is completely continuous Telomerase activity is needed...
Sanger sequencing allows the identification of the bases and their order in a target piece of DNA. This technique exploits the way DNA is transcribed by using a primer and allowing new DNA to be created from its 3' end. In this technique, DNA is manufactured in vitro, which means that enzymes and molecules are combined in a test tube to allow the reactions to occur. In the original technique, four tubes were prepared, each containing the same reactants, but...
please help with all three!
For DNA synthesis to take place in a Sanger sequencing reaction, the DNA polymerase must catalyze a reaction between the 3'- the last nucleotide and the 5- of the next nucleotide to be added. hydroxyl group, phosphate group O phosphate group, hydroxyl group O hydroxyl group, hydroxyl group O phosphate group, phosphate group QUESTION 6 millions of individual DNA fragments are isolated and sequenced in parallel during each machine run. O traditional whole-genome sequencing O...
How has DNA-sequencing technology evolved in response to the emerging needs of genome scientists? Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Not all terms will be used. Reset Help reduce speed sequencing methods, a single serves as a template. In one such strategy, the DNA polymerase is tethered in a nanopore and fluorescently labeled nucleotides are detected as they are incorporated. The overall goal is to and accuracy of sequencing...
In DNA sequencing, ddNTPs differ from dNTPs in that ddNTPs are
lacking a OH at the
a.
2' carbon
b.
5' carbon
c.
3' carbon
please answer as many as you can!! I am low on questions and
could use the help!
Mother || Child II Male 1 Male 2 Results from a paternity test using DNA fingerprinting is shown. DNA was isolated from a mother, her child and 2 potential fathers. Primers designed to amplify different satellite DNA regions...
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