Which of the following make(s) sequencing by the Sanger chain-termination method possible? (Selec...
Which of the following make(s) sequencing by the Sanger chain-termination method possible? (Select all that apply.)12.17
a. Complementary single-stranded nucleic acid sequences can come together to form a duplex molecule.
b. Single-stranded nucleic acid molecules can be immobilized on certain types of filter paper.
c. A DNA strand whose 3' end terminates in a dideoxynucleotide cannot be elongated.
d. Duplex nucleic acid molecules can be separated by size by means of electrophoresis.
e. New nucleotides are added only to the 3' end of a growing DNA strand.
Homework Answers
Answer: Option C, E.
Explanation:
- During sanger sequencing, dNTP's are added in 5'-->3' direction.
- The elongation is terminated when ddNTP's is added to the growing chain.
- This terminated sequence is denatured from the template strand and run on electrophoresis setup.
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