The Sanger method of DNA sequencing uses which chain-terminating ingredient to separate fragments differing by a...
The Sanger method of DNA sequencing uses which chain-terminating ingredient to separate fragments differing by a single nucleotide
a. Magnesium b. Heat c. ddNTPs d. restriction enzymes e. none of the above
Homework Answers
Answer: c) ddNTPs
Explanation:
ddNTPs are dideoxynucleotides, they do not have the 3’-OH group which is essential for the formation of phosphodiester bond between the nucleotides, and as a result the nucleotide chain formation is terminated whenever the ddNTP is encountered by the DNA polymerase. Thus the Sanger method uses ddNTPs as chain terminating ingredient, which results in formation of variable lengths of DNA fragments.
Normally the ddNTPs will be fluorescently or radioactively labelled to detect their presence. The concentration of ddNTPs added will be much lower than the normal deoxynucleotides so that enough number of DNA fragments would be formed
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The Sanger method of DNA sequencing uses which chain-terminating
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Please explain how to identify which lane corresponds to which ddNTP. 44. For the Sanger sequencing...
Please explain how to identify
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