All of the following are DNA sequencing techniques except___. Select all that apply
A) pyrosequecing
B) mullis chain terminating sequencing
c) sanger dideoxy sequencing
d) DNA fingerprinting
e) Illumina sequencing
Correct answers would be :
B) mullis chain terminating sequencing
d) DNA fingerprinting
Reason: All of the mentioned options except B and D are DNA sequencing methods.Mullis chain terminating sequencing does not exist and DNA fingerprinting is not a sequencing method.
All of the following are DNA sequencing techniques except___. Select all that apply A) pyrosequecing B)...
The Sanger method of DNA sequencing uses which chain-terminating ingredient to separate fragments differing by a single nucleotide a. Magnesium b. Heat c. ddNTPs d. restriction enzymes e. none of the above
24. Which three of the following six features are common to Illumina and Sanger sequencing technologies (you will get 1/3 for each correct answer and minus 1/3 for each incorrect answer): Select one or more: a. Both technologies use terminators b. Both methods require PCR amplification c. Both methods require a ligation step d. Both methods require a restriction endonuclease e. Both methods require primers f. Both methods were developed by scientists while under the influence of strong hallucinogenic drugs...
Which of the following is true regarding DNA sequencing? (multiple answers possible) a. Next generation sequencing is limited to simultaneous sequencing of 23 different nucleotide chains at a time b. Next generation sequencing or “sequencing by synthesis”, requires ddNTPs c. DNA fragments generated by the Sanger sequencing reaction each contain a primer incorporated at the 5’ end of the nucleotide chain. d. Primers are labeled with different flurophores allowing all 4 chain termination reactions for Sanger sequencing to be combined...
Which of the following is true regarding Illumina sequencing? Select all that apply. The fluorescent label remains on the DNA strand as additional nucleotides are added. Each of the nucleotides has a different colored label. The blocking group is removable. A blocking group is present on the 2' OH of the nucleotide.
DNA Sequencing Techniques Compare/contrast Sanger sequencing, pact-bio, and oxford nanopore sequencing techniques. Include how to make a library, and why you would use each one in respect to cost, size, % error, etc. Consider budget, what you want to get out of it, and is there merit to combine different technologies together?
Q1) List the limitations and challenges of dideoxy termination method of DNA sequencing? Q2) In the context of mutation detection, describe the differences between first- and second-generation sequencing? Q3) 6-year-old child presenting with sensorineural deafness, sequencing of MYO15A gene by Sanger method was performed and showed the following chromatogram: A- Describe the principle of Sanger sequencing method? B- Describe the DNA change: position, type of variant and genotype?
Which of the following answer(s) are true (select all that apply)? a)PCR results in exclusively the desired region to be amplified. b) PCR primers must be outside the region of interest. c) PCR primers must be flanking the region of interest. d) Sequencing primers must be flanking the region of interest. e) All of the nucleotides used in Sanger Sequencing would also be able to be used for a PCR reaction where you are trying to amplify a gene of...
Which of the following is not true regarding both Sanger and Illumina sequencing? Group of answer choices a) Both require DNA polymerase b) Both utilize both standard dNTPs and non-standard dNTPs c) Both require a primer d) All of the above are true for both types of sequencing
Question 2
Classical DNA sequencing is a powerful technique. Consider a situation where you first sequence a normal strand of DNA, then sequence a mutated strand. When analysing the results of sequencing reactions, run on a gel, would you be able to distinguish each of the following mutation types, compared to unmutated sequence, using classic "Sanger" sequencing? A silent mutation An insertion of 6 nucleotides A sense mutation Which of the following factors directly breaks phosphodiester bonds? Select all that...
In automated DNA sequencing reaction by the Sanger method, which of the following is NOT a component of the reaction? A) fluorescently-labeled deoxynucleotides B) DNA polymerase C) a DNA primer D) the DNA to be sequenced E) none of the above (i.e. all the above are required in the reaction)