Which of the following is not true regarding both Sanger and Illumina sequencing? Group of answer...
Which of the following is not true regarding both Sanger and Illumina sequencing?
Group of answer choices
a) Both require DNA polymerase
b) Both utilize both standard dNTPs and non-standard dNTPs
c) Both require a primer
d) All of the above are true for both types of sequencing
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Which of the following is not true regarding
both Sanger and Illumina sequencing?
Group of answer...
24. Which three of the following six features are common to Illumina and Sanger sequencing technologies...
24. Which three of the following six features are common to Illumina and Sanger sequencing technologies (you will get 1/3 for each correct answer and minus 1/3 for each incorrect answer): Select one or more: a. Both technologies use terminators b. Both methods require PCR amplification c. Both methods require a ligation step d. Both methods require a restriction endonuclease e. Both methods require primers f. Both methods were developed by scientists while under the influence of strong hallucinogenic drugs...
In automated DNA sequencing reaction by the Sanger method, which of the following is NOT a...
In automated DNA sequencing reaction by the Sanger method, which of the following is NOT a component of the reaction? A) fluorescently-labeled deoxynucleotides B) DNA polymerase C) a DNA primer D) the DNA to be sequenced E) none of the above (i.e. all the above are required in the reaction)
Which of the following is true regarding DNA sequencing? (multiple answers possible) a. Next generation sequencing...
Which of the following is true regarding DNA sequencing? (multiple answers possible) a. Next generation sequencing is limited to simultaneous sequencing of 23 different nucleotide chains at a time b. Next generation sequencing or “sequencing by synthesis”, requires ddNTPs c. DNA fragments generated by the Sanger sequencing reaction each contain a primer incorporated at the 5’ end of the nucleotide chain. d. Primers are labeled with different flurophores allowing all 4 chain termination reactions for Sanger sequencing to be combined...
Which of the following is true regarding Illumina sequencing? Select all that apply. The fluorescent label...
Which of the following is true regarding Illumina sequencing? Select all that apply. The fluorescent label remains on the DNA strand as additional nucleotides are added. Each of the nucleotides has a different colored label. The blocking group is removable. A blocking group is present on the 2' OH of the nucleotide.
Q A) All dNTPs in Illumina sequencing by synthesis are labeled with a fluorescent dye, True...
Q A) All dNTPs in Illumina sequencing by synthesis are labeled with a fluorescent dye, True OR False B) The fundamental principles on which Evolution is based are genetic variation and natural selection. True OR False C) A group of species that includes the common ancestor and all descendants of that ancestor is the definition of which of the following terms? A, paraphyletic group B polyphyletic group C morphophyletic group D monophyletic group D) One way to construct phylogenetic trees...
A scientist is doing a classical Sanger sequencing reaction. He sets up four reaction tubes, labeling...
A scientist is doing a classical Sanger sequencing reaction. He sets up four reaction tubes, labeling them G, A, T, and C. To each tube he adds template, DNA polymerase, buffer, primer, all four dNTPs, and accidently, small amounts of all four ddNTPs. He then runs his samples on a sequencing gel. What do you think the gel will look like? The G, A, T, and C lanes will all look like normal, and it will still be easy to...
Q1) Innovations that make SMRT DNA sequencing possible are . Group of answer choices A) Phospholinked...
Q1) Innovations that make SMRT DNA sequencing possible are . Group of answer choices A) Phospholinked nucleotides B) Single molecule sequencing C) ZMW D) Long read lengths E) All of the Above Q2) Why is it important to attach adaptors and amplify the DNA pieces on the glass slide before Illumina Sequencing by Synthesis? Group of answer choices A) To attach the DNA pieces to the glass slide. B)To distinguish DNA pieces from each other. C) To amplify each sequence...
Which of the following answer(s) are true (select all that apply)? a)PCR results in exclusively the...
Which of the following answer(s) are true (select all that apply)? a)PCR results in exclusively the desired region to be amplified. b) PCR primers must be outside the region of interest. c) PCR primers must be flanking the region of interest. d) Sequencing primers must be flanking the region of interest. e) All of the nucleotides used in Sanger Sequencing would also be able to be used for a PCR reaction where you are trying to amplify a gene of...
All of the following are DNA sequencing techniques except___. Select all that apply A) pyrosequecing B)...
All of the following are DNA sequencing techniques except___. Select all that apply A) pyrosequecing B) mullis chain terminating sequencing c) sanger dideoxy sequencing d) DNA fingerprinting e) Illumina sequencing
please help with all three! For DNA synthesis to take place in a Sanger sequencing reaction,...
please help with all three!
For DNA synthesis to take place in a Sanger sequencing reaction, the DNA polymerase must catalyze a reaction between the 3'- the last nucleotide and the 5- of the next nucleotide to be added. hydroxyl group, phosphate group O phosphate group, hydroxyl group O hydroxyl group, hydroxyl group O phosphate group, phosphate group QUESTION 6 millions of individual DNA fragments are isolated and sequenced in parallel during each machine run. O traditional whole-genome sequencing O...
please help with all three!
For DNA synthesis to take place in a Sanger sequencing reaction, the DNA polymerase must catalyze a reaction between the 3'- the last nucleotide and the 5- of the next nucleotide to be added. hydroxyl group, phosphate group O phosphate group, hydroxyl group O hydroxyl group, hydroxyl group O phosphate group, phosphate group QUESTION 6 millions of individual DNA fragments are isolated and sequenced in parallel during each machine run. O traditional whole-genome sequencing O...
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