Question

24. Which three of the following six features are common to Illumina and Sanger sequencing technologies (you will get 1/3 for each correct answer and minus 1/3 for each incorrect answer):

Select one or more:

a. Both technologies use terminators

b. Both methods require PCR amplification

c. Both methods require a ligation step

d. Both methods require a restriction endonuclease

e. Both methods require primers

f. Both methods were developed by scientists while under the influence of strong hallucinogenic drugs

25. Which three of the following six statements about Illumina sequencing are true (you will get 1/3 for each correct answer and minus 1/3 for each incorrect answer):

Select one or more:

a. it can read both forward and reverse strands in a single run

b. Sequencing is not performed using reversable terminators

c. Illumina sequencing does not use single DNA molecules

d. Each nucleotide emits a different colour

e. DNA fragments are not bound to a flow cell

f. Illumina sequencing does not requires a PCR amplification step

26. Which three of the following six statements about Illumina sequencing are true (you will get 1/3 for each correct answer and minus 1/3 for each incorrect answer):

Select one or more:

a. Illumina sequencing has a lower error rate than Sanger sequencing

b. DNA sequences to be read are amplified by 'bridge PCR'

c. PCR amplification occures in microreactors

d. Sequencing occures on a 'flow cell'

e. Flourescent-labelled reversible terminators are used

f. Illumina sequencing produces longer fragement reads than Sanger sequencing

Answer 1

24. Which three of the following six features are common to Illumina and Sanger sequencing technologies

answer:

a. Both technologies use terminators

c. Both methods require a ligation step

e. Both methods require primers

25. Which three of the following six statements about Illumina sequencing are true

answer:

b. Sequencing is not performed using reversable terminators

c. Illumina sequencing does not use single DNA molecules

f. Illumina sequencing does not requires a PCR amplification step

26. Which three of the following six statements about Illumina sequencing are true

answer:

a. Illumina sequencing has a lower error rate than Sanger sequencing

d. Sequencing occures on a 'flow cell'

f. Illumina sequencing produces longer fragement reads than Sanger sequencing