Question

18. Which THREE of the following six statements are true (you get 1/3 for each correct answer and minus 1/3 for each incorrect answer):

Select one or more:

a. GFP was originally found in algae

b. GFP binds strongly and specifically to actin

c. The original GFP gene has been artificially mutated to produce other colours

d. CFP stands for Coral Fluorescent Protein

e. Genes such as GFP can replace a native coding sequence to act as a reporter protein

f. GFP stands for Green Fluorescent Protein

19. Which TWO of the following six statements are NOT true (you get 1/2 for each correct answer and minus 1/4 for each incorrect answer):

Select one or more:

a. Biotin can act as a hapten.

b. Haptens are small molecules recognised by antibodies

c. Nucleic acids labelled with radioactive isotopes such as 32P can be specifically recognised by antibodies.

d. Biotin (Vitamin B7) is specifically recognised (bound to) by streptavidin.

e. Digoxygenin is a hapten derived from the death cap mushroom (Amanita phalloides)

f. Fluorescein is a fluorescent hapten

20. Which THREE of the following six statements are true (you get 1/3 for each correct answer and minus 1/3 for each incorrect answer):

Select one or more:

a. The nucleotides Adenine and Uracil form a stable complementary base pair

b. The nucleotides Guanine and Cytosine form a stable complementary base pair

c. The nucleotides Guanine and Adenine form a stable complementary base pair

d. The nucleotides Adenine and Thymine form a stable complementary base pair

e. The nucleotides Cytosine and Uracil form a stable complementary base pair

f. The nucleotides Uracil and Thymine form a stable complementary base pair

21. Which ONE of the following statements is false:

Select one:

a. Microsatellites are usually typed by PCR and size fractionation in an automated sequencer

b. The repeat unit in a microsatellite is usually between 2 and 6 bases long

c. One of the main mechanisms of minisatellite mutation is unequal crossing over

d. Y chromosome variation can be useful in forensic cases of sexual assault

e. The repeat unit in a minisatellite is usually between 11 and 60 bases long

f. Tetranucleotide repeat microsatellites can occur in the coding regions of genes

g. One of the main mechanisms of microsatellite mutation is slipped-strand mispairing

h. If a VNTR has three alleles, there are six possible genotypes

Answer 1

18. b, c and f

Explanation