In plaque assay, why is soft agar used in the top layer? Why is it important...
Homework Answers
The plaque assay will help determine the individual of virus. The overlay assist agar helps to keep the cells stable. It also helps to limit the spread of virus. So that when virus lyse a bacterial cell and comes out, it infects the neighboring cells and plaques could be formed.
The bacterial is exponential phase carry out active metabolism and rapid cell division. The secondary metabolites, bacterial defense systems and bacterial cell are young and easy to manipulate. Exponential phase cells are prone to easier viral attacks, stress sensible and sensitive to antibiotics. Hence this is the ideal phase to study viral plaque assay.
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plaque assay, why is soft agar used in the top layer?
Why is it important...
You repeat the viral plaque assay adding 10 microliters of viral solution to 990 microliters of...
You repeat the viral plaque assay adding 10 microliters of viral solution to 990 microliters of LB. You perform this serial dilution five times. You add 10 microliters from each dilution to the corresponding host bacteria molten top agar mixture which is then spread onto an LB agar plate. The next day you count 39 plaques on the fourth plate. Why is it that on plate 3 the number of plaques will be 39 x 10^2 and on plate 5...
In Chapter 21, we performed the plaque assay in which the T4 bacteriophage was used to...
In Chapter 21, we performed the plaque assay in which the T4 bacteriophage was used to infect E. coli strain B cells. Select one of the scenarios below, indicate the expected result, and describe what could have caused the observed result. You are asked to repeat the plaque assay using the T4 bacteriophage with E. coli strain B. After a 24 hour incubation period at 37°C, you observe no plaques on all of your plates. You are asked to repeat...
QUESTION 6 Why is agar used in lab, give important properties of agar AND doncribe why...
QUESTION 6 Why is agar used in lab, give important properties of agar AND doncribe why it would be important to separate two species of bacteria on by spreading the across a plate as was done in the lab dass TTT Arial 3(126 T E . .
A microbiology student was carrying out an independent research project in which he needed to know...
A microbiology student was carrying out an independent research
project in which he needed to know the number of infectious phage
particles added during a particular step in an experimental
protocol. In preparation for the experiment, he passed a crude
mixture of phage plus infected bacteria through a 0.45 micrometer
filter and collected the filtrate (portrayed below in the
green-capped Eppendorf tube):
Once the filtrate was prepared, the student used the P-20 shown
in the photograph to transfer a volume...
Why is agar used in lab, give 5 important properties of agu AND describe why it...
Why is agar used in lab, give 5 important properties of agu AND describe why it would be important to separate two species of bacteria on by spreading them across a plate as was done in the lab clans TTT Ariel (120) T E
I got half credit for this question and not sure what I got wrong. Please do...
I got half credit for this question and not sure what I got
wrong. Please do the math and explain.
A microbiology student was carrying out an independent research project in which he needed to know the number of infectious phage particles added during a particular step in an experimental protocol. In preparation for the experiment, he passed a crude mixture of phage plus infected bacteria through a 0.45 micrometer filter and collected the filtrate (portrayed below in the green-capped...
QUESTION 4 Why is media such as Sabouraud Dextrose Agar (SDA) used for the growth of...
QUESTION 4 Why is media such as Sabouraud Dextrose Agar (SDA) used for the growth of yeast and fung? Choose all that apply. lower pH inhibits growth of bacteria is differential for bacteria and fungi/yeasts QUESTIONS The difference between MBC and MIC is that... the MBC is the highest concentration of antibiotic that kills the organism will the MIC is the lowest concentration of antibiotic that kills the organism. The MBC is the lowest concentration of antibiotic that inhibits bacterial...
1. What is the purpose of Thin-Layer Chromatography (TLC)? 2. What are the phases involved in...
1. What is the purpose of Thin-Layer Chromatography (TLC)? 2. What are the phases involved in TLC? 3. What is coated on the TLC plate? 4. What is the purpose of lining the wall of the TLC chamber with paper? 5. Why is it important to use a small amount of the mobile phase? 6. Why should you remove the TLC plate before the mobile phase reaches the top? 7. What type of compounds are visible with UV light? 8....
Would the methylene chloride layer be above or below the experiment? Justify your answer. 1. aqueous layer in today...
Would the methylene chloride layer be above or below the experiment? Justify your answer. 1. aqueous layer in today's ium carbonate used in the isolation of caffeine? Be specific as to the 2. Why is potass chemical species the carbonate may act on. Why was sodium sulfate used? 3. 4. After introducing 1.0 g of potassium carbonate into the centri hot water extract, it was capped, shaken, and then cooled to room temperature. Following this, roug minute. Why wasn't the...
plz help the inoculating loop After you use an inoculating loop to transfer bacteria from a...
plz help
the inoculating loop After you use an inoculating loop to transfer bacteria from a culture tube onto a slide, you should before putting it away For the toolbar, press ALT+F10 (PC) or ALT-FN-F10 (Mac). TTTT Paragraph Arial 3 (12pt) E - T %D0Q TT. Пнти се QIX I Path: P Words:0 QUESTION 3 In lab, bacterial cultures (Petri plates and tubes of culture broth) should be kept covered at all times. This is partly to protect us from...
QUESTION 6 Why is agar used in lab, give important properties of agar AND doncribe why it would be important to separate two species of bacteria on by spreading the across a plate as was done in the lab dass TTT Arial 3(126 T E . .
A microbiology student was carrying out an independent research
project in which he needed to know the number of infectious phage
particles added during a particular step in an experimental
protocol. In preparation for the experiment, he passed a crude
mixture of phage plus infected bacteria through a 0.45 micrometer
filter and collected the filtrate (portrayed below in the
green-capped Eppendorf tube):
Once the filtrate was prepared, the student used the P-20 shown
in the photograph to transfer a volume...
Why is agar used in lab, give 5 important properties of agu AND describe why it would be important to separate two species of bacteria on by spreading them across a plate as was done in the lab clans TTT Ariel (120) T E
I got half credit for this question and not sure what I got
wrong. Please do the math and explain.
A microbiology student was carrying out an independent research project in which he needed to know the number of infectious phage particles added during a particular step in an experimental protocol. In preparation for the experiment, he passed a crude mixture of phage plus infected bacteria through a 0.45 micrometer filter and collected the filtrate (portrayed below in the green-capped...
QUESTION 4 Why is media such as Sabouraud Dextrose Agar (SDA) used for the growth of yeast and fung? Choose all that apply. lower pH inhibits growth of bacteria is differential for bacteria and fungi/yeasts QUESTIONS The difference between MBC and MIC is that... the MBC is the highest concentration of antibiotic that kills the organism will the MIC is the lowest concentration of antibiotic that kills the organism. The MBC is the lowest concentration of antibiotic that inhibits bacterial...
1. What is the purpose of Thin-Layer Chromatography (TLC)? 2. What are the phases involved in TLC? 3. What is coated on the TLC plate? 4. What is the purpose of lining the wall of the TLC chamber with paper? 5. Why is it important to use a small amount of the mobile phase? 6. Why should you remove the TLC plate before the mobile phase reaches the top? 7. What type of compounds are visible with UV light? 8....
Would the methylene chloride layer be above or below the experiment? Justify your answer. 1. aqueous layer in today's ium carbonate used in the isolation of caffeine? Be specific as to the 2. Why is potass chemical species the carbonate may act on. Why was sodium sulfate used? 3. 4. After introducing 1.0 g of potassium carbonate into the centri hot water extract, it was capped, shaken, and then cooled to room temperature. Following this, roug minute. Why wasn't the...
plz help
the inoculating loop After you use an inoculating loop to transfer bacteria from a culture tube onto a slide, you should before putting it away For the toolbar, press ALT+F10 (PC) or ALT-FN-F10 (Mac). TTTT Paragraph Arial 3 (12pt) E - T %D0Q TT. Пнти се QIX I Path: P Words:0 QUESTION 3 In lab, bacterial cultures (Petri plates and tubes of culture broth) should be kept covered at all times. This is partly to protect us from...
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