Why are next-generation DNA sequencing technologies known as sequencing-by-synthesis? Why are next-generation DNA sequencing technologies known...
Why are next-generation DNA sequencing technologies known as sequencing-by-synthesis? Why are next-generation DNA sequencing technologies known as sequencing-by-synthesis?
The complete DNA strands are synthesized then sequenced.
Numerous synthesized fragments of DNA are sequenced to determine which nucleotides were incorporated.
The sequencing occurs during S phase of the cell cycle.
RNA molecules are synthesized off of the DNA templates and the incorporated nucleotides are then determined.
Incorporated nucleotides are determined while they are being added to a growing DNA strand.
Homework Answers
Answer: Option 5 is
correct
Explanation:
Sequencing by synthesis: During Illumina sequencing, each
nucleotide is differentially labeled with a specific dye that emits
a particular wavelength that can be detected.
As the new strand is synthesized, the emission is recorded to
determine its sequence.
Add Answer to:
Why are next-generation DNA sequencing technologies known as
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Which of the following is true regarding DNA sequencing? (multiple answers possible) a. Next generation sequencing...
Which of the following is true regarding DNA sequencing? (multiple answers possible) a. Next generation sequencing is limited to simultaneous sequencing of 23 different nucleotide chains at a time b. Next generation sequencing or “sequencing by synthesis”, requires ddNTPs c. DNA fragments generated by the Sanger sequencing reaction each contain a primer incorporated at the 5’ end of the nucleotide chain. d. Primers are labeled with different flurophores allowing all 4 chain termination reactions for Sanger sequencing to be combined...
Only answers please don’t show work 33) Viral DNA incorporated into host cell DNA is known...
Only answers please don’t show work
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How has DNA-sequencing technology evolved in response to the emerging needs of genome scientists? Drag the...
How has DNA-sequencing technology evolved in response to the emerging needs of genome scientists? Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Not all terms will be used. Reset Help reduce speed sequencing methods, a single serves as a template. In one such strategy, the DNA polymerase is tethered in a nanopore and fluorescently labeled nucleotides are detected as they are incorporated. The overall goal is to and accuracy of sequencing...
The following diagram represents a replication bubble associated with DNA synthesis. Based on this diagram, select...
The following diagram represents a replication bubble associated
with DNA synthesis. Based on this diagram, select all of the
options below that are true.
1 | 2 ---- Quadrants 1 and 4 are associated with lagging strand synthesis Quadrants 1 and 4 are associated with leading strand synthesis Quadrants 1 and 2 are associated with lagging strand synthesis Quadrants 3 and 4 are associated with lagging strand synthesis Synthesis of both daughter strands is completely continuous Telomerase activity is needed...
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please help with all three! For DNA synthesis to take place in a Sanger sequencing reaction,...
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For DNA synthesis to take place in a Sanger sequencing reaction, the DNA polymerase must catalyze a reaction between the 3'- the last nucleotide and the 5- of the next nucleotide to be added. hydroxyl group, phosphate group O phosphate group, hydroxyl group O hydroxyl group, hydroxyl group O phosphate group, phosphate group QUESTION 6 millions of individual DNA fragments are isolated and sequenced in parallel during each machine run. O traditional whole-genome sequencing O...
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44. During DNA replication, the double helix is unwound, creating two single strands. One of these is called the strand, on which the progress of DNA Polymerase III is – 45. The other strand is called the strand, on which the synthesis is 16. During DNA replication, synthesis of a new sequence on the Leading Strand is continuous. Why is synthesis on the Lagging Strand discontinuous? 47. Generation of Okazaki fragments proceeds in three steps. Indicate which of the following...
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Only answers please don’t show work
33) Viral DNA incorporated into host cell DNA is known as a/an A) phage. B) envelope C) capsid. 33) 34) How is transformation in bacteria most A) the creation of a strand of RNA from DNA molecule B) the type of semiconservative by DNA C) the infection of cells by a phage DNA molecule D) the e of a strand of DNA from an RNA molecule E) assimilation of external DNA into a cell...
How has DNA-sequencing technology evolved in response to the emerging needs of genome scientists? Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Not all terms will be used. Reset Help reduce speed sequencing methods, a single serves as a template. In one such strategy, the DNA polymerase is tethered in a nanopore and fluorescently labeled nucleotides are detected as they are incorporated. The overall goal is to and accuracy of sequencing...
The following diagram represents a replication bubble associated
with DNA synthesis. Based on this diagram, select all of the
options below that are true.
1 | 2 ---- Quadrants 1 and 4 are associated with lagging strand synthesis Quadrants 1 and 4 are associated with leading strand synthesis Quadrants 1 and 2 are associated with lagging strand synthesis Quadrants 3 and 4 are associated with lagging strand synthesis Synthesis of both daughter strands is completely continuous Telomerase activity is needed...
please help with all three!
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44. During DNA replication, the double helix is unwound, creating two single strands. One of these is called the strand, on which the progress of DNA Polymerase III is – 45. The other strand is called the strand, on which the synthesis is 16. During DNA replication, synthesis of a new sequence on the Leading Strand is continuous. Why is synthesis on the Lagging Strand discontinuous? 47. Generation of Okazaki fragments proceeds in three steps. Indicate which of the following...
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