Which of the following statements is FALSE about Southern blotting? a) smaller nucleic acid fragments move...
Which of the following statements is FALSE about Southern blotting?
a) smaller nucleic acid fragments move faster than larger nucleic acid fragments in gels
b) a hybridization probe for Southern blot can be single stranded DNA or RNA
c) In most cases, DNA is cut with a restriction enzyme prior to gel electrophoresis
d) smaller hybridization probes will always detect smaller DNA fragments than larger probes
e) Southern blotting is used to analyze DNA
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Which of the following statements is FALSE about Southern
blotting?
a) smaller nucleic acid fragments move...
14. Restriction endonucleases are a. enzymes that restrict DNA synthesis b. enzymes that cut DNA in...
14. Restriction endonucleases are a. enzymes that restrict DNA synthesis b. enzymes that cut DNA in specific sequences c. nuclear proteins that are involved in transcription d. components of the ribosomes involved in protein synthesis 15. The first step in southern blotting is a. converting DNA into RNA b. cutting high molecular weight DNA into smaller pieces c. converting RNA into DNA d. radioactively labeling the DNA so it can be detected after the procedure is complete 16. The major...
9. An alteration in the nucleic acid sequence in which a specific restriction endonuclease cuts can...
9. An alteration in the nucleic acid sequence in which a specific restriction endonuclease cuts can be detected by a. ELISA b. flow cytometry c. RFLP d. FISH 10. Which of the following techniques enables the identification of a particular sequence of nucleic acid on a cell or tissue? a. northern blot b. western Blot c. FISH d. southern blot 11. The polymerase chain reaction is used to a. convert RNA back into DNA b. amplify a target nucleic acid...
QUESTION 6 Assume you are studying a protein-coding gene, ACEX, which includes 4 exons as illustrated...
QUESTION 6 Assume you are studying a protein-coding gene, ACEX, which includes 4 exons as illustrated in the gene map below. The 5' UTR and 3' UTR segments are each 25 bp long. Exons 1 thru 4 are 100, 200, 300, 400 bp long, respectively. Each intron is 200 bp each. The locations of the relevant EcoRI sites within the ACEX locus are indicated, but the location of other restriction enzyme sites (like BamHI) are not shown." EcoRI probe EcoRI...
The picture above represents an agarose gel that was used to analyze plasmid DNA after it...
The picture above represents an agarose gel that was used to analyze plasmid DNA after it was cut with the restriction enzyme HindIll. The plasmid was incubated with Hindill until all of the available Hindlll cut sites were cut by HindIll. After running the sample on the gel, three bands were detected (Note that there are three wells shown at the top of the gel for loading samples, however, only the middle well was loaded with sample). Based on this...
Questions 9-12 deal with SSR loci in Sam's genome. Remember that SSRs (simple sequence repeats) are...
Questions 9-12 deal with SSR loci in Sam's genome. Remember that SSRs (simple sequence repeats) are tandem (next to each other) repeats of short sequences (a few bp). Such tandem repeats are located at specific sites scattered throughout the genome. Individuals vary in the number of repeats at each site sites can have up to 100 repeats. Each locus with the same repeats is called an SSR ocus DNA was extracted from Sam's white blood cells and cut with a...
A good enzyme for use in an immunoassay should have a: A. rapid degradation rate. B....
A good enzyme for use in an immunoassay should have a: A. rapid degradation rate. B. high substrate conversion rate. C. high cost. D. toxic enzymatic product. What is the basis of strand displacement amplification? A. Two different primers bind to the same strand of DNA. B. Two different primers each bind to complementary strands of DNA. C. Multiple primers bind to fragmented DNA targets. D. Primers are joined together. For a probe to hybridize to the target, which condition...
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been...
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been isolated and purified, but its amino acid sequence has not been determined. We wish to clone the gene for protein P. (a) How can a probe be prepared to identify the gene for protein P? (b) If we have prepared a radioactive messenger RNA as our probe in part (a), how could we verify that it is the mRNA for protein P? (c) If...
Figure 21 CO ah--o-o __ d. HCOO-C-CHE . HC- cy 18) In Figure 2.1, which is...
Figure 21 CO ah--o-o __ d. HCOO-C-CHE . HC- cy 18) In Figure 2.1, which is an alcohol? B) b A) a 9c D) E) e Figure 23 HONCHO -CH- OH 19) What kind of bond is at the arrow in Figure 2.37 A) hydrogen bond B) ionic bond C) peptide bond D) double covalent bond E) disulfide bridge 20) Starch, dextran, glycogen, and cellulose are polymers of A) amino acids. B) acids. C) nucleic acids. D) glucose. E) fatty...
QUESTION 6 Assume you are studying a protein-coding gene, ACEX, which includes 4 exons as illustrated in the gene map below. The 5' UTR and 3' UTR segments are each 25 bp long. Exons 1 thru 4 are 100, 200, 300, 400 bp long, respectively. Each intron is 200 bp each. The locations of the relevant EcoRI sites within the ACEX locus are indicated, but the location of other restriction enzyme sites (like BamHI) are not shown." EcoRI probe EcoRI...
The picture above represents an agarose gel that was used to analyze plasmid DNA after it was cut with the restriction enzyme HindIll. The plasmid was incubated with Hindill until all of the available Hindlll cut sites were cut by HindIll. After running the sample on the gel, three bands were detected (Note that there are three wells shown at the top of the gel for loading samples, however, only the middle well was loaded with sample). Based on this...
Questions 9-12 deal with SSR loci in Sam's genome. Remember that SSRs (simple sequence repeats) are tandem (next to each other) repeats of short sequences (a few bp). Such tandem repeats are located at specific sites scattered throughout the genome. Individuals vary in the number of repeats at each site sites can have up to 100 repeats. Each locus with the same repeats is called an SSR ocus DNA was extracted from Sam's white blood cells and cut with a...
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been isolated and purified, but its amino acid sequence has not been determined. We wish to clone the gene for protein P. (a) How can a probe be prepared to identify the gene for protein P? (b) If we have prepared a radioactive messenger RNA as our probe in part (a), how could we verify that it is the mRNA for protein P? (c) If...
Figure 21 CO ah--o-o __ d. HCOO-C-CHE . HC- cy 18) In Figure 2.1, which is an alcohol? B) b A) a 9c D) E) e Figure 23 HONCHO -CH- OH 19) What kind of bond is at the arrow in Figure 2.37 A) hydrogen bond B) ionic bond C) peptide bond D) double covalent bond E) disulfide bridge 20) Starch, dextran, glycogen, and cellulose are polymers of A) amino acids. B) acids. C) nucleic acids. D) glucose. E) fatty...
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