1) Suppose that gene A 3,000 bp. Suppose that g contained within intron 1 opposite directions...
5. Consider the wild-type F8 gene. What would happen to the 1) transcript (mRNA) sequence and quantity, 2) to the protein (sequence, quantity, function) and 3) the person’s overall phenotype if they were homozygous (both copies of the gene are the same) for a 3 base-pair deletion in the: i. First intron ii. First exon iii. The promoter iv. 5’UTR v. Last exon 6. What environmental factor could affect the phenotype caused by an F8 null mutation? Thrombophilia is a...
TranslationOverview:The purpose of this activity is to help the students to understand how replication, transcription, and translation are connected. Students will use a sequence from a bacterial gene that confers resistance to antibiotics (carbapenems). They will be asked to apply the knowledge obtained in the class lecture to (1) find the promoter in the sequence, (2) determine the amino acid sequence of a fragment of the polypeptide, (3) "reverse translate" a fragment of the polypeptide, and (4) identify mutations in...
Question 2: a) You determine that xpd is expressed preferentially in muscle. Your colleague wants to know if any of the mutations affect the stability of the RNA transcript, thereby leading to higher turnover and lower overall mRNA levels. What techniques could you use to test her hypothesis? List the pros and cons of each method. (hint: try to think of at least three methods you could use) b) You find that your collegue is correct, and that the xpda...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...