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how yo distinguish between the Mycobacterium phlei to Mycobacterium smegmatis. please explain clearly how to distinguish

how yo distinguish between the Mycobacterium phlei to Mycobacterium smegmatis. please explain clearly how to distinguish
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Answer #1

The distinguishing features of M.phlei and M.smegmatis are:


M.phlei shows growth at 52 degrees whereas M.smegmatis shows no growth at this temperature.
M.phlei shows no growth on methyl violet dye whereas M.smegmatis shows 100% growth in this dye.
M.phlei do not change the color of MacConkey agar whereas M.smegmatis changes the color of MacConkey agar.
M.phlei cannot produce acids from glucose, mannose, galactose,arabinose,xylose,rhamnose,inositol,mannitol and sorbitol whereas M.smegmatis can produce acids from glucose,mannose,galactose,arabinose,xylose,rhamnose,inositol,mannitol and sorbitol.


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