Homework Answers
Yes , ONPG enters through the Lac permease.
Lac Operon genes consists of 3 structural genes which are lacZ,lacYand lacA.LacZ encodes for enzyme called βgalactoside .
LacY encodes for a membrane protein called lactose permease which helps in the import of lactose across cell membrane.
β-galactoside helps to convert ONPG to glucose and ortho-nitrophenol.ONPG is colourless whereas Ortho nitrophenol is slightly yellow in colour.
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In the Biochemical Analysis of the Lac Operon experiment, ONPG is cleaved to give a yellow...
You have systematically mutagenized the lac operon in E. coli to produce a mutation that disrupts...
You have systematically mutagenized the lac operon in E. coli to produce a mutation that disrupts the function of each of the following elements: a. the promoter for LacI (P(I)) b. the LacI gene c. CRP binding site d. the promoter for the lac operon (P(lac)) e. the operator sequence f. a mutation in lacZ that disrupts the coding region but does not disrupt transcription g. a mutation in lacZ that blocks transcription For each of the above mutations, what...
1. Describe the lac operon and its regulation. Figures can be helpful for darity, but are...
1. Describe the lac operon and its regulation. Figures can be helpful for darity, but are not required. 2. Describe the wild-type E. coli phenotype for beta-galactosidase expression in glucose-rich, lactose-rich, and glucose/lactose-rich environments. 3. What is ONPG, and why is it used to determine the levels of beta-galactosidase activity? 4. Explain the control(s) used in the experiment. 5. Explain any sources of error in this experiment: if the experiment didn't go as planned, give at least one reason why...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the follow...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the following mutations: lacZ, lacY, laď·0; and lach. For each of these six strains, describe the phenotype you would observe using the following assays. Explain your answers. [Notes: (1) IPTG is a colorless synthetic molecule that acts as an inducer of lac operon expression but cannot serve as a carbon source for bacterial growth because it...
Most of what we know about the lac operon in E. coli has come from the...
Most of what we know about the lac operon in E. coli has come from the genetic analysis of various mutants. Below is a list of mutants for regions of the operon. A + superscript indicates no mutation and normal function of that region, - indicates a knock out and no function of that region, c indicates the mutation resulted in constitutive action of that region. The effect of the mutation is determined by expression of the lacZ gene as...
Catabolite repression can regulate the expression of the lac operon in E. coli. In terms of...
Catabolite repression can regulate the expression of the lac operon in E. coli. In terms of this positive regulation of the lac operon: What is the activator? Would it work in cis or trans? What molecule binds to the allosteric domain of the activator? What influences strongly the levels of the molecule you answered for part b? To what regulatory sequence does the activator bind? What part/domain of the activator? What is the effect of this binding? Name the two...
In the table below, predict (yes or no) whether or not the E. coli lac operon...
In the table below, predict (yes or no) whether or not the E. coli lac operon will be transcriptionally active in the presence or absence of glucose or lactose as indicated and respond to questions "a" and "b." (20 points) Lactose Glucose Lac expression? No Yes NO Yes Yes NO Yes No YES 1) Explain each of your answers in terms of the molecular mechanisms that are known to underlie the regulation of the lac operon. 2) Which mechanism is...
Which of the following situations will give the highest level of lac operon transcription in E....
Which of the following situations will give the highest level of lac operon transcription in E. coli? **this was my question from my professor there WAS NO additional information given to me. I assume the answer is no glucose and positive for lactose. I think the answer is B; i just wanted to check. A. no glucose no lactose B. no glucose plus lactose C. glucose no lactose D.All conditions will result in the same level of transciption Please explain...
PLEASE help answer all of question 18 a-e 18. What would the lac operon look like in a eukaryote? List two possible changes in chromatin. Explain them to each other. a. b. Transcriptional control...
PLEASE help answer all of question 18 a-e
18. What would the lac operon look like in a eukaryote? List two possible changes in chromatin. Explain them to each other. a. b. Transcriptional control. Draw how a single gene (Lacz) would be transcribed and regulated c. If they are each in different chromosomes, draw how their expression would be coordinated Processing control. Pick one approach and explain to your partners d. Translational regulation. Pick one approach and explain to your...
Briefly explain your answers to the following questions. A mutation (Mutation A) in the lac operon...
Briefly explain your answers to the following questions. A mutation (Mutation A) in the lac operon of E. coli leads to an inability to ferment lactose, and the expression of the operon is always off. Introduction into the mutant of an F' factor containing the wild type lac operon does NOT restore the ability to ferment lactose, i.e., there is no expression of the operon from the plasmid either. a. What is the probable nature of Mutation A? Is the...
4. Explain how the genes that control galactose metabolism in yeast are regulated in yeast. How does it differ from the regulation of the lac operon in bacteria? please be detailed in your answer
4. Explain how the genes that control galactose metabolism in yeast are regulated in yeast. How does it differ from the regulation of the lac operon in bacteria? please be detailed in your answer
1. Describe the lac operon and its regulation. Figures can be helpful for darity, but are not required. 2. Describe the wild-type E. coli phenotype for beta-galactosidase expression in glucose-rich, lactose-rich, and glucose/lactose-rich environments. 3. What is ONPG, and why is it used to determine the levels of beta-galactosidase activity? 4. Explain the control(s) used in the experiment. 5. Explain any sources of error in this experiment: if the experiment didn't go as planned, give at least one reason why...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the following mutations: lacZ, lacY, laď·0; and lach. For each of these six strains, describe the phenotype you would observe using the following assays. Explain your answers. [Notes: (1) IPTG is a colorless synthetic molecule that acts as an inducer of lac operon expression but cannot serve as a carbon source for bacterial growth because it...
PLEASE help answer all of question 18 a-e
18. What would the lac operon look like in a eukaryote? List two possible changes in chromatin. Explain them to each other. a. b. Transcriptional control. Draw how a single gene (Lacz) would be transcribed and regulated c. If they are each in different chromosomes, draw how their expression would be coordinated Processing control. Pick one approach and explain to your partners d. Translational regulation. Pick one approach and explain to your...
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