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Catabolite repression can regulate the expression of the lac operon in E. coli.   In terms of...

  1. Catabolite repression can regulate the expression of the lac operon in E. coli.   In terms of this positive regulation of the lac operon:
    1. What is the activator? Would it work in cis or trans?
  1. What molecule binds to the allosteric domain of the activator?
  1. What influences strongly the levels of the molecule you answered for part b?
  1. To what regulatory sequence does the activator bind? What part/domain of the activator?
  1. What is the effect of this binding?

  1. Name the two scientists who discovered the lac operon.

  1. Describe the lac operon as it would be in the presence of lactose but absence of glucose. (Do not simply give the level of transcription but explain what is going on with each kind of regulation to arrive at that level.)

                                                         

  1. A lac Oc mutation is cis-acting. Explain what this means
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