Homework Answers
a. inactive, inactive, medium
b. active, inactive, off
c. active, inactive, off
d. inactive, inactive, leaky
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Consider an E. coli strain carrying a mutation in crp (encoding the catabolite activator protein) that...
Consider what you know about the lac operon, and predict whether the repressor and the activator...
Consider what you know about the lac operon, and predict whether the repressor and the activator is active (able to bind to DNA) or inactive (unable to bind to DNA), and the level of transcription in the following scenarios. Options for transcription levels include high, medium, leaky and off. a. When lactose is present but glucose is not, the repressor is and the activator is leading to levels of transcription. and the activator is b. When lactose and glucose are...
The gene machine program shows you what happens when lactose is present in E. coli, and...
The gene machine program shows you what happens when lactose is present in E. coli, and how the lac operon is under negative control. However, the lac operon is also under positive control from a protein called CRP, eAMP Receptor Protein. The absence of the lac repressor is essential but not sufficient for effective transcription of the lac operon. RNA polymerase also depends on the presence of CRP. Like the lac repressor, which can bind to the DNA and lactose....
1) The lac operon is… a) Positive repressible b) Both negative inducible and positive repressible c)...
1) The lac operon is… a) Positive repressible b) Both negative inducible and positive repressible c) Positive inducible d) Negative inducible 2) An activator regulatory protein… a) Is involved in negative regulatory control b) Is a cis acting regulatory element c) Recruits RNA polymerase to the promoter d) Prevents RNA polymerase from binding the promoter 3) For the lac operon, the presence of the substrate (lactose) activates the repressor. True or False? a) true b) false 4) cAMP… a) Activates...
26. The lac operon in E. coli consists of genes that code for enzymes necessary for...
26. The lac operon in E. coli consists of genes that code for enzymes necessary for the breakdown of lactose. When lactose is absent, the operon is inactive because a repressor protein binds to a specific site in the lac operon. When lactose is present, lactose molecules bind to the repressor protein, causing the repressor protein to dissociate from the binding site. In the absence of glucose (a preferred energy source for bacteria), the protein CAP binds to a regulatory...
Catabolite repression can regulate the expression of the lac operon in E. coli. In terms of...
Catabolite repression can regulate the expression of the lac operon in E. coli. In terms of this positive regulation of the lac operon: What is the activator? Would it work in cis or trans? What molecule binds to the allosteric domain of the activator? What influences strongly the levels of the molecule you answered for part b? To what regulatory sequence does the activator bind? What part/domain of the activator? What is the effect of this binding? Name the two...
Question 12 2 pts What is the role of CAMP in regulation of the lactose operon?...
Question 12 2 pts What is the role of CAMP in regulation of the lactose operon? CAMP is a repressor of the lactose operon and turns off gene expression of the lactose operon CAMP is an activator and prevents the repressor protein from binding to the operator region of the operon which allows for the lactose operon to be turned "on". 5 CAMP is an inducer of the lactose operon and also an activator since it prevents the repressor protein...
consider a strain of E. coli that has a mutation in the gene encoding the IIA...
consider a strain of E. coli that has a mutation in the gene encoding the IIA protein of the phosphotransferase system. The transport and phosphorylation of glucose is not affected by this mutation and the phosphate group is transferred to glucose as in the wildtype strain. Also, the interaction between the mutant IIA protein and Lacy is not affected by the mutation. However, the mutation does affect the IIA protein's interactions with adenylate cyclase and the phosphorylated form of the...
genetics An E. Coli strain possesses the following genotype: F(P+)/" pCo+z+y+ where l' is a mutation...
genetics
An E. Coli strain possesses the following genotype: F(P+)/" pCo+z+y+ where l' is a mutation that eliminates the production of repressor and PC eliminates the ability of the polymerase to bind the promoter. The structural genes will be transcribed both in the presence and absence of lactose O will be transcribed only if lactose is absent O will be transcribed only if lactose is present will not be transcribed under any onditions will be transcribed but not translated
B2. Consider E. coli cells, each having one of the following mutations: a) a mutant lac...
B2. Consider E. coli cells, each having one of the following mutations: a) a mutant lac operator (Oc locus) that cannot bind repressor. b) a mutant lac repressor (I- gene product) that cannot bind to the lac operator. c) a mutant lac repressor (the Is gene product) that cannot bind to lactose. d) a mutant lac promoter that cannot bind CAP + cAMP. What effect would each mutation have on the function of lac operon (assuming no glucose is present)...
1) Which of the following conditions is most likely to cause the lactose operon to be...
1) Which of the following conditions is most likely to cause the lactose operon to be transcribed? A) There is more glucose in the cell than lactose. B) There is glucose but no lactose in the cell. C) The cyclic AMP and lactose levels are both high within the cell. D) The CAMP level is high and the lactose level is low. 2) How does the transcription of structural genes in an inducible operon occur? A) It occurs continuously in...
Consider what you know about the lac operon, and predict whether the repressor and the activator is active (able to bind to DNA) or inactive (unable to bind to DNA), and the level of transcription in the following scenarios. Options for transcription levels include high, medium, leaky and off. a. When lactose is present but glucose is not, the repressor is and the activator is leading to levels of transcription. and the activator is b. When lactose and glucose are...
The gene machine program shows you what happens when lactose is present in E. coli, and how the lac operon is under negative control. However, the lac operon is also under positive control from a protein called CRP, eAMP Receptor Protein. The absence of the lac repressor is essential but not sufficient for effective transcription of the lac operon. RNA polymerase also depends on the presence of CRP. Like the lac repressor, which can bind to the DNA and lactose....
26. The lac operon in E. coli consists of genes that code for enzymes necessary for the breakdown of lactose. When lactose is absent, the operon is inactive because a repressor protein binds to a specific site in the lac operon. When lactose is present, lactose molecules bind to the repressor protein, causing the repressor protein to dissociate from the binding site. In the absence of glucose (a preferred energy source for bacteria), the protein CAP binds to a regulatory...
Question 12 2 pts What is the role of CAMP in regulation of the lactose operon? CAMP is a repressor of the lactose operon and turns off gene expression of the lactose operon CAMP is an activator and prevents the repressor protein from binding to the operator region of the operon which allows for the lactose operon to be turned "on". 5 CAMP is an inducer of the lactose operon and also an activator since it prevents the repressor protein...
consider a strain of E. coli that has a mutation in the gene encoding the IIA protein of the phosphotransferase system. The transport and phosphorylation of glucose is not affected by this mutation and the phosphate group is transferred to glucose as in the wildtype strain. Also, the interaction between the mutant IIA protein and Lacy is not affected by the mutation. However, the mutation does affect the IIA protein's interactions with adenylate cyclase and the phosphorylated form of the...
genetics
An E. Coli strain possesses the following genotype: F(P+)/" pCo+z+y+ where l' is a mutation that eliminates the production of repressor and PC eliminates the ability of the polymerase to bind the promoter. The structural genes will be transcribed both in the presence and absence of lactose O will be transcribed only if lactose is absent O will be transcribed only if lactose is present will not be transcribed under any onditions will be transcribed but not translated
1) Which of the following conditions is most likely to cause the lactose operon to be transcribed? A) There is more glucose in the cell than lactose. B) There is glucose but no lactose in the cell. C) The cyclic AMP and lactose levels are both high within the cell. D) The CAMP level is high and the lactose level is low. 2) How does the transcription of structural genes in an inducible operon occur? A) It occurs continuously in...
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