Question

consider a strain of E. coli that has a mutation in the gene encoding the IIA protein of the phosphotransferase system. The transport and phosphorylation of glucose is not affected by this mutation and the phosphate group is transferred to glucose as in the wildtype strain. Also, the interaction between the mutant IIA protein and Lacy is not affected by the mutation. However, the mutation does affect the IIA protein's interactions with adenylate cyclase and the phosphorylated form of the mutant IIA no longer stimulates adenylate cyclase. Show how the lac operon will be expressed in this mutant strain of E. coli in Table 2 using the same rating system you employed for Table 1. Table 2 Growth Condition IIA Mutant glucose/lactose activation repression lacZ expression +/+ +/- 7+