4) (2 pts) Explain what will happen to an E. coli culture in the following media:...
4) (2 pts) Explain what will happen to an E. coli culture in the following media: a. No glucose + no lactose (1 pt) b. Glucose + lactose but the cells have a supper repressor mutation (1 pt)
5) The lacZ gene codes for a protein in the lac operon. What is this protein and name its function? (2 pts)
Homework Answers
When glucose is absent then cAMP concentration increase, cAMP bind with CAP protein and this complex bind with promoter , but lactose is absent so repressor bind with operator , so operon is off in this moment .
Super suppressor mutation means , repressor protein can't bind with inducer(lactose) , repressor always bind with operator irrespective of the medium . So operon is always off.
LacZ encode beta galactosidase protein which can cleave beta1,4 glycosidic bond of lactose (glucose +galactose) . By this enzyme E.coli can utilise lactose as energy source.
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4) (2 pts) Explain what will happen to an E. coli culture in the
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for
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Yet, all the cells in your body contain the same
genes (and same alleles). The difference across cell types
is that genes get selectively expressed (turned on or off)
based on the proteins needed for cellular function given their
environment.
Select which statement explains the reason why hair does not
normally grow on your muscle cells.
a.
Muscle cells have the gene for keratin, but do not express
it
b.
Muscle cells do not have the gene for keratin and...
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