Question

A laboratory mouse has a particular locus in its genome that contains an A-T nuclutide pair at particular site.this mouse accedently escaped its gage ,found its way to the chemical cabinet ,and got exposed to some dimethylsulfate ,provide a close up drawing of the hydrogen-bonding situation that ultimately led to the mutation?please give me the answer in details with explanation how you analyses the question to get the answer ??!!!

Answer 1

Dimethyl sulfate (DMS) with the chemical formula CH3-O-SO2-O-CH3 is used as a methylating agent. Dimethyl sulfate causes selective methylation of nucleophilic sites. Methylation by DMS occurs mostly with guanine producing N7-methylguanine. Adenine and cytosine are also methylated by DMS producing N3- and N7-methyladenine and methyl cytosine. N3 methyladenine blocks DNA replication. In addition, depurination and base mispairing by both 3-methyladenine and 3-methylcytosine lead to mutagenesis by DMS. This is explained in detail in the uploaded image.

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