Question

A member of my research group recently performed protein-ligand binding experiments. She used UV-Vis to determine θ by monitoring the PLL The data she collected is provided below: 6. L mM 0.1 .0060 0.2 .0124 0.3 .0190 0.4 .0245 0.5 .0307 0.6 .0380 0.7 .0430 0.8 0481 0.9 0530 1.0 0591 40.0865 2.0 3.0 .170 4.0 227 5.0 283 7.5 10.0 .500 15.0 .640 20.0 721 30.0 .812 50.0 60.0 70.0 80.0 90.0 100 150 200 .889 .905 .917 0.927 935 .941 960 .970 .112 420 (a) Generate a protein-ligand binding curve. Based on its appearance does the binding event appear to be cooperative. Explain. (b) Use a Hill Plot to confirm or contradict your assessment. Provide your reasoning. (15 points)

Answer 1

4 0.60206 0.227 -0.532153637 971 0.283-0403732721 7.510875061| -0.42| -0.140178703 3 3 5 0.698 Log[S] 16 | || 15 1.176091 0.64 0.249877473 0.721 0.412331061 30 1.477121 0.812 0.63539818 40 1.602060.865 0.806682339 50 1698970.889 0.903578782 L91 201 1.3 | 0103 360 1.778151 0 0.9050.978924974 70 1.845098 0.917 1.043291243 80 1.90309 0.927 1.103756874 0.935 1.157898254 2 0.941 1.202737612 8150 2.1760910.96 1.380211242 2.301031 0.37し 1.509650481 90 1.954243 100 2