Question

A student is working in a research lab studying the glucose transporter and evolution of this trans-membrane protein. There is genetic evidence that this transporter may be present in ancient bacteria but so far no functional data. The student designs an experiment where the cells are grown in a monolayer on a semi-permeable membrane on O-rings then placed in a chamber where 13C glucose flux (rate of transport/unit area) is monitored. Experiment is setup to monitor flux of glucose into cells. From data of flux versus glucose concentration, how can the student tell if the process is mediated or non- mediated transport? (Hint competitive inhibitors and chemicals to inactive the transporter are available)

Answer 1

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