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1. (20 points) Using the HH equation, calculate the total negative charge on the phosphate groups of ATP at pH 7.0 given that the pKas for the three of the four ionizable OH groups in phosphocreatine have a pKa of 2.5 and the fourth has a pKa of 6.5.

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Total neagtive charge on the phosphate group is -3.756alassmate Date -0 Pk 5-70 S-7o

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