Question

The restriction map below shows the MstII sites in a portion of the beta^a-globin allele. The beta^s allele lacks the internal MstII site. Genomic DNA has been isolated from several individuals, cut with MstII, separated by electrophoresis, transferred to nylon membrane, and probed with the labeled 1.4 kb MstII fragment. Following results are obtained from five individuals. Based on the southern data, predict the genotype of the following individuals which may either be beta^a/beta^a, or beta^a/beta^s, or beta^s/beta^s, or may carry other gross abnormalities in the beta-globin gene.

Answer 1

1 β^a/ β^s

2 abnormal gene (none of the higher sizes obtained)

3 abnormal gene (0.2 kb missing)

4 β^s/ β^s

5 abnormal gene (0.2 kb missing)