Why is a protein crystal necessary to resolve protein structures via X-ray? (as opposed to a solution of molecules? As opposed to a single protein that say is completely static?)
Protein X-ray crystallography is a technique used to obtain the three-dimensional structure of a particular protein by x-ray diffraction of its crystallized form. This three dimensional structure is crucial to determining a protein's functionality. Making crystals creates a lattice in which this technique aligns millions of proteins molecules together to make the data collection more sensitive. It's like getting a stack of papers, measuring the width with a ruler, and dividing that length with the number of pages to determine the width of one piece of paper. By this averaging technique, the noise level gets reduced and the signal to noise ratio increases. The specificity of the protein's active sites and binding sites is completely dependent on the protein's precise conformation. X-ray crystallography can reveal the precise three-dimensional positions of most atoms in a protein molecule because x-rays and covalent bonds have similar wavelength, and therefore currently provides the best visualization of protein structure.
Why is a protein crystal necessary to resolve protein structures via X-ray? (as opposed to a...
X-ray crystal structures of CIF30 and BrF30 have been determined. (a) Would you expect the lone pair on the center halogen to be axial or equatorial in these molecules? Why? (b) Which molecule would you predict to have the smaller Fequatorial-central atom-oxygen angle? Explain your reasoning.
Which of the following statements is true about X-ray diffraction? a) X-ray diffraction can be used for capturing protein dynamics b) the structure obtained from X-ray diffraction can contain crystal-packing artifacts c) X-ray diffraction can be used to obtain structures of intrinsically disordered proteins d) The x-ray beam is scattered primarily by the nuclei of atoms
X-ray diffraction operates at wavelengths of ~1 A in order deliver Angstrom-resolution protein structures. [4] Explain, why and how NMR spectroscopy, which operates at much longer, radiofrequency wavelengths (10 ^m), can deliver protein structures of comparable (Angstrom) resolution. As an NMR spectroscopes, do you prefer larger or smaller values for the duration of the 90degrees ovules for the target nucleus/channel (e.g. for 1H)? Justify.
An x-ray diffraction experiment involves a beam that is incident onto a single crystal. As the angle of incidence is decreased from 90°, the first strong interference is found at 73°. At what angle would the next interference occur?
Which technique, X-ray diffraction or NMR spectroscopy gives a more realistic rendition of a protein structure (assume that the protein is amenable to characterization by either method, ie it is small enough to resolve by NMR, it forms reasonable crystals etc). You could make a case for either technique, so justify your answer.
Protein crystallography essay: please write a detailed essay with diagrams on X-ray crystallography. Your answer must include: (A) the typical strategy of determining a three dimensional structure of a macromolecule using X-ray crystallography. (B) how protein crystallography is used in a drug design pipeline and discuss why protein crystallography is useful for the development of new therapeutics or the optimisation of existing pharmaceuticals (C) the major steps involved in using X-ray crystallography for drug design (D) The advantages and disadvantages/limitations...
The X-Ray crystal structure of the α-terpinene-N-phenylmaleimide Diels-Alder adduct was obtained. Several stereodrawings of the structure generated are provided in your lab manual 4) a. Is the adduct obtained the exo- or endo- adduct? EXO or ENDO (circle one) b. The phenyl (CoHs) group bonded to the nitrogen is often written as a cyclohexatriene with alternating double/single bonds. Does the crystal structure support the existence of alternating double and single bonds (yes or no)? (Hint: Compare the bond angles and...
Explain why it is necessary to conduct vibrational tests for structures Q5 (a) (5 marks) The block shown in Figure Q5 has a mass m and is supported on the solid ground by a spring with stiffness k and a parallel viscous damper with damping constant d. An out of balance force F(t) is exerted on the block. Suclh force F(t) is approximated by a harmonic function causing a steady state displacement x(t) shown in Figure Q5 The following relationship...
(a) Differentiate between Face- Centered Cubic (FCC) and Body-Centered Cubic (BCC) crystal structures. Why FCC metals are more ductile than BCC metals? 5 marks) (ii) show the relationship between the unit cell edge length, a, and the atomic radius, R, for a BCC crystal. Iron has a BCC crystal structure, an atomic radius of 0.124 nm, and atomic weight of 55.85 g/mol. Calculate its theoretical density Given: Avogardo's Number is 6.02 x 105 atoms/mol (5 marks) Figure 1 Determine the...
1. [6.4% ) Write down the electron configuration for Br. 2. [6.4 %) Draw resonance structures for (a) NO:, (b) NNO (a) N-NEO (b) NN-o 3. [6.4%) In which pair do both compounds exhibit predominantly covalent bonding? A. BaCl and H-O B. NaBr and SO C. K O and NBr D. CCl and Asl 4. [6.4 % ) Rank the following hydrocarbons in order of increasing vapor pressure: CHe, CvoH, CH, CH16, C22H 5. [6.4%) O and N2 in air...