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5) (3 pts) For a double-stranded DNA molecule, which of the following statements are true? If...
Shown below are the sequences of three duplex DNA molecules. I. 5" AGCCTAACTGCGAT 3' II. 5' CGCATTATTGTCAA 3' III. 5' CTAACCCGCACTGG 3' 3' TCGGATTGACGCTA 5' 3' GCGTAATAACAGTT 5' 3' GATTGGGCGTGACC 5' a. State the relationship between the percentage of AT base pairs in a double-stranded DNA molecule and the melting point of that molecule. b. Order the above three molecules (I, II, III) in terms of their melting points from lowest to highest. The graph below shows the results of...
For the following single-stranded DNA molecule: 3’ – G T A C A A G T C A – 5’ 1. Write the complementary strand, being sure to label polarity. (2 pts) 2. What is the GC content for the resulting double-stranded DNA molecule? (2 pts)
Given the following base compositions, determine which double-stranded DNA molecule is expected to be the most thermodynamically stable? ■A + T = 10% ■A + T = 30% ■G + C = 60% ■G + C = 50% G + C = 40% Please explain!!
The partial sequence of one strand of a double stranded DNA molecule is: 5’ ---GACGAAGTGCTGCAGAAAGTCCGCGTTATAGGCATGAATTCCTGAGG--- 3’ Write the sequence of both strands of the DNA fragment when this DNA is cleaved with both EcoRI and PstI. The top of your duplex DNA fragment should be derived from the standard sequence given above.
1. Very simple toy model for base-pairing of double stranded DNA. (This problem is originally due to Kittel.) DNA is an important biological heteropolymer. A single DNA molecule base-pairs with a complementary DNA molecule to form a duplex double stranded structure. Consider two strands of complementary DNA. Each strand has N monomers. Each monomer is cross- linked by base-pairing to the corresponding monomer on the complementary DNA molecule. Suppose that the energy for breaking a cross link is є and...
A double-stranded DNA molecule of 175 base pairs long had (A+T/(G+C) = 0.75. How many adenine nucleotides are there in this DNA? 75 37.5 150
1. Answer the following questions concerning a section of double-stranded DNA containing 1000 base pairs. a. If the DNA contains 28% adenosine residues, how many guanosine residues are in the DNA? b. Are the nucleotide compositions of each of the two individual strands of DNA identical? Explain. c. If the DNA is entirely in the B-DNA conformation: i. How many helical turns does the DNA contain? ii. What is the length of the DNA? 2. Write the sequence of a...
Which of the statement is true? If a double-stranded DNA molecule of 125 million base pairs were scaled to the size of a human hair, it would have a diameter of 0.003 inches and be 1 mile long. If this scaled-up DNA molecule were condensed and compacted into a chromosome, into which of the objects below could that chromosome fit? A. a tennis ball B. a bathtub C. a pencil eraser D. a soda (pop) can E. a basketball
1. In the following double-stranded DNA sequences, ___ has the lowest melting temperature and __ has the highest melting temperature. 1) GATCG 2) GGCCG 3) AATTA 4) AATCA CTAGC CCGGC TTAAT TTAGT 2. In linear nucleic acids, nucleotides are bonded through their ___ and ___ carbon atoms. 3. Although RNA and DNA are similar in structure, one difference is that ___. DNA uses the base adenine RNA uses the base cytosine the RNA sugar...
QUESTION 9 Strand invasion requires a__, O A. 5', single-stranded B. 5', double-stranded ° C. 3, single-stranded O D.3', double-stranded DNA molecule. QUESTION 10 The form of genetic recombination that allows movement of genetic elements from one DNA site to another is termed: O A site-specific recombination O B. homologous recombination ° C. branch migration D.transposition QUESTION 9 Strand invasion requires a__, O A. 5', single-stranded B. 5', double-stranded ° C. 3, single-stranded O D.3', double-stranded DNA molecule. QUESTION 10...