Homework Answers
The extra chromosomal circular double stranded DNA [DS-DNA] present in bacterial cell is know as plasmid DNA.
Reason for isolating plasmid DNA-
Plasmid DNA are considered as best cloning vector in gene cloning process by biotechnologist because plasmids are
1- easily isolated and purified from micro organism like Bacteria and viruses.
2- easily introduced in to host cell or cloning organism.
3- capacity of independent replication contain ori gene (replication origin) and transmission in cloning organism.
4- small in size i.e. less than 10Kb for easy handling.
5- presence of palindromic sequences as in desired DNA.
6- containing suitable marker genes [like tetracycline resistance, ampicillin resistance and kanamycin resistance].
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1. What are some reasons for isolating plasmid DNA? 2. What are the functions of sodium...
1. Why does the plasmid you are purifying have the beta-lactamase gene? 2. You are using...
1. Why does the plasmid you are purifying have the beta-lactamase gene? 2. You are using a small column to bind DNA molecules during purification of the plasmid DNA. This column binds DNA via ionic interactions. What is the charge on the column which allows it to bind DNA? Answer positive" or "negative and explain your answer. 3. Where is the plasmid at the END of each of the following steps? Circle the letter of the correct answers for each...
18 15 16 17 7- What is the concentration of DNA whereby a 1:100 dilution has...
18 15 16 17 7- What is the concentration of DNA whereby a 1:100 dilution has an observance reading of 0.015 at 260 nm? a. 6 ug mL b. 60 ug/mL c. 75 ug/mL d. 750 ug/mL 8- When measuring the concentration of RNA by spectrophotometry at 260 nm, the absorbance reading is multiplied by the dilution and a conversion factor of a. 20 6.30 c. 40 d. 50 9-DNA is isolated from a clinical sample. The absorbance at 260...
1. Fill in the table above with what you observe on your plates. 2. Bacterial transformation...
1. Fill in the table above with what you observe on your
plates.
2. Bacterial transformation occurred on which agar plate (s)?
What evidence do you have that the bacteria were transformed
here?
3. Which plates have glowing growth? Explain what causes
bacteria to glow.
II. Transformation of E. coli with Plasmid DNA (PGLO) 1. Three LB (Luria Broth) agar plates are obtained. The plates contain: • Plate A: LB-Agar/Ampicillin/Arabinose • Plate B: LB-Agar/Ampicillin • Plate C: LB-Agar 2. Three...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteina...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
lab question 1. What is the basis of the different purification methods? 2. What are some...
lab question 1. What is the basis of the different purification methods? 2. What are some of the factors the might have interfered with your results? 3. How might you improve the process to increase the yield and purity? lab process E. coli BL21 (DE3) cells were transformed with the pET Topo-1521 vector containing a reading frame encoding the green fluorescent protein (GFP). Cells were cultured in M9ZB media at 37°C until the absorbance at 600 nm reached 0.7, at...
The smallest chemical units of matter are atoms b) molecules c) protons d) neutrons e) electrons...
The smallest chemical units of matter are atoms b) molecules c) protons d) neutrons e) electrons . Which of the following would have the largest size? a) an atom b) a molecule c) a proton d) a neutron e) an electron 3. Isotopes of an element differ in the number of a) protons in the nucleus b) electrons in the nucleus © neutrons in the nucleus d) electron clouds e) energy levels they contain 4. VO The atomic number represents...
10. Write a one-page summary of the attached paper? INTRODUCTION Many problems can develop in activated...
10. Write a one-page summary of the attached paper? INTRODUCTION Many problems can develop in activated sludge operation that adversely affect effluent quality with origins in the engineering, hydraulic and microbiological components of the process. The real "heart" of the activated sludge system is the development and maintenance of a mixed microbial culture (activated sludge) that treats wastewater and which can be managed. One definition of a wastewater treatment plant operator is a "bug farmer", one who controls the aeration...
1. Why does the plasmid you are purifying have the beta-lactamase gene? 2. You are using a small column to bind DNA molecules during purification of the plasmid DNA. This column binds DNA via ionic interactions. What is the charge on the column which allows it to bind DNA? Answer positive" or "negative and explain your answer. 3. Where is the plasmid at the END of each of the following steps? Circle the letter of the correct answers for each...
18 15 16 17 7- What is the concentration of DNA whereby a 1:100 dilution has an observance reading of 0.015 at 260 nm? a. 6 ug mL b. 60 ug/mL c. 75 ug/mL d. 750 ug/mL 8- When measuring the concentration of RNA by spectrophotometry at 260 nm, the absorbance reading is multiplied by the dilution and a conversion factor of a. 20 6.30 c. 40 d. 50 9-DNA is isolated from a clinical sample. The absorbance at 260...
1. Fill in the table above with what you observe on your
plates.
2. Bacterial transformation occurred on which agar plate (s)?
What evidence do you have that the bacteria were transformed
here?
3. Which plates have glowing growth? Explain what causes
bacteria to glow.
II. Transformation of E. coli with Plasmid DNA (PGLO) 1. Three LB (Luria Broth) agar plates are obtained. The plates contain: • Plate A: LB-Agar/Ampicillin/Arabinose • Plate B: LB-Agar/Ampicillin • Plate C: LB-Agar 2. Three...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
The smallest chemical units of matter are atoms b) molecules c) protons d) neutrons e) electrons . Which of the following would have the largest size? a) an atom b) a molecule c) a proton d) a neutron e) an electron 3. Isotopes of an element differ in the number of a) protons in the nucleus b) electrons in the nucleus © neutrons in the nucleus d) electron clouds e) energy levels they contain 4. VO The atomic number represents...
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