Question

3. In this experiment, both the tube and the LB-only plates were used as controls to help us validate our transformation results. The left column depicts four different problems or human errors (unknown to the researcher at the time of the experiment) that are possible with this experiment. The right hand side shows four hypothetical experimental results, cach result is the consequence of one error or problem listed on the Jeft. Correctly match the letter (A-D) of the experimental results with the correct error or problem. Problem or Error Experimental results A. LB Plasmid DNA liquid added to T rube mus really 9596 ethanol (tocic to bacteria). LB-Amp LB LB-Amp 0 0 Control All E. coli culrures had already acquired resistance to Ampicillin prior to transformation 00 c. LB LB-Amp LB LB-Amp Hear shock step set to 95°C, instead of 42°C, killing all cells Forgor to add CaCl, to make cells competent prior to transformation. Control controll o

Answer 1

Problems are written below with their respective experimental results

Plasmid DNA liquid added to T tube was really 95% ethanol( toxic to bacteria)- D

All E.coli cultures had already acquired resistance to Ampicillin prior to transformation-B

Heat shock set up to 95-degree Celcius, instead of 42-degree Celcius, killing all cells-A

Forgot to add CaCl2 to make cells competent prior to transformation-C